Identification and characterization of endothelin binding sites in rat renal papillary and glomerular membranes.
Identification and characterization of endothelin binding sites in rat renal papillary and glomerular membranes.
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大鼠肾乳头和肾小球膜内皮素结合位点的鉴定和表征。
DOI：
10.1016/0006-291x(89)91972-4
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发表时间：
1989
影响因子：
3.1
通讯作者：
Ballermann,BJ
中科院分区：
文献类型：
--
作者：
Martin,ER;Marsden,PA;Brenner,BM;Ballermann,BJ
本研究旨在鉴定和鉴定大鼠肾乳头和肾小球膜上的特定内皮素结合部位，这些部位似乎是这种新的多肽激素的靶组织。饱和结合研究表明，这些结合位点具有很高且均一的亲和力。乳头膜和肾小球膜的解离常数分别为662±151和1309±123 pM，受体密度分别为7666±920和5831±348 fmol/mg蛋白。在100~300 pm范围内，ET-1、ET-3和沙拉毒素均可抑制[125I]-ET与IC50‘S的结合，而血管紧张素II、心钠素和血小板衍生生长因子等无关多肽不能竞争[125I]-ET结合。内皮素1中羧基末端色氨酸的缺失使其与肾小球结合部位的亲和力降低了2个数量级。当pH>8时，[~(125)I]-ET与肾小球小球膜结合的特异性最强，当[~(125)I]-ET共价连接到肾小球膜结合部位时，经放射自显影后的SDS-PAGE显示明显的45 kDa的特异性标记条带。这条带代表的是内皮素受体(S)的一个亚单位、受体本身，还是细胞内的内皮素结合蛋白，还有待确定。
The present study was designed to identify and characterize specific endothelin binding sites in membranes of rat renal papillae and glomeruli which appear to be target tissues for this new peptide hormone. Saturation binding studies indicate that the sites have a high and uniform affinity. The dissociation constants averaged 662 ± 151 and 1309 ± 123 pM and the receptor densities 7666 ± 920 and 5831 ± 348 fmol/mg protein for papillary and glomerular membranes, respectively. Endothelin 1, endothelin 3 and sarafotoxin all inhibited [125I]-endothelin binding with IC50' s in the 100–300 pM range, whereas unrelated peptides, namely angiotensin II, atrial natriuretic peptide, and platelet-derived growth factor failed to compete for [125I]-endothelin binding. Deletion of the carboxyterminal tryptophan in endothelin 1 reduced its affinity for glomerular binding sites by 2 orders of magnitude. Specific endothelin binding to these membranes was maximal at pH 4 and was markedly inhibited as the pH was raised above 8. When [125I]-endothelin is covalenthy linked to glomerular membrane binding sites, SDS-PAGE of these solubilized membranes followed by autoradiography reveals a predominant specifically labeled band of 45 kDa. Whether this band represents a subunit of the endothelin receptor(s), the receptor proper, or an intracellular endothelin binding protein remains to be determined.
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培养的非恶性和恶性人类乳腺上皮细胞的克隆增殖。
影响因子：
11.2
作者：
Smith,HS;Lan,S;Ceriani,R;Hackett,AJ;Stampfer,MR
通讯作者：
Stampfer,MR
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影响因子：
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作者：
R. Buick';T. Stanisic;S. Fry;S. Salmon;J. Trent;P. Krasovich
通讯作者：
P. Krasovich
DOI：
--
发表时间：
--
期刊：
影响因子：
--
作者：
通讯作者：
--
电离辐射后人类乳腺细胞的存活。
DOI：
10.1080/09553008314550821
发表时间：
1983
期刊：
International journal of radiation biology and related studies in physics, chemistry, and medicine
影响因子：
--
作者：
Cathers,LE;Gould,MN
通讯作者：
Gould,MN