Microbacterium helvum sp. nov., a novel actinobacterium isolated from cow dung

Microbacterium helvum sp. nov., a novel actinobacterium isolated from cow dung
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微杆菌属

DOI:
10.1007/s00203-021-02311-9
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发表时间:
2021-02
期刊:
Archives of Microbiolog
影响因子:
--
通讯作者:
Wensheng Xiang
Wensheng Xiang
中科院分区:
其他
文献类型:
--
作者:
Xiao Li;Lida Zhang;Fuyan Huang;Junwei Zhao;Han Wang;Yanjie Jiao;Xilang Yang;Xiangjing Wang;Wensheng Xiang

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从中国东北黑龙江省尚志市牛粪中分离到一株革兰氏阳性、需氧、不运动、不产芽孢、短杆状的菌株NEAU-LLCT。菌落呈浅黄色,圆形,边缘完整。菌株NEAU-LLCT在15-45 ℃和pH 6.0-10.0下生长。NaCl浓度范围为0 - 5%(W/V)。NEAU-LLCT的16 SrRNA基因序列与庆和微杆菌JCM 18735 T(98.5%)、溶孢微杆菌JCM 1358 T(98.3%)和济州微杆菌JCM 18734 T(98.2%)的同源性较高。全细胞糖为葡萄糖、鼠李糖和核糖。甲基萘醌含有MK-12和MK-13。细胞壁水解产物中的氨基酸主要为鸟氨酸、谷氨酸、赖氨酸和少量的丙氨酸、甘氨酸。主要脂肪酸为异C16:0、异C18:0、反异C15:0和反异C17:0。主要极性脂质为二磷脂酰甘油、磷脂酰甘油和一种未鉴定的糖脂。NEAU-LLCT基因组全长4,369,375 bp,G + C含量为70.28mol%。结合DNA-DNA杂交结果和一些表型特征,证明菌株NEAU-LLCT可以与其近缘菌株区分开来。因此,认为菌株NEAU-LLCT代表一个新种,命名为瑞士微杆菌(Microbacterium helvum sp.)(模式菌株NEAU-LLCT= CCTCC AA 2018026 T = JCM 32661 T)。
A Gram-positive, aerobic, non-motile, non-spore-forming, short rod-shaped strain, NEAU-LLCT, was isolated from cow dung in Shangzhi City, Heilongjiang Province, Northeast China and identified by a polyphasic taxonomic study. Colonies was light yellow, round, with entire margin. Strain NEAU-LLCTwas grown at 15-45 ℃ and pH 6.0-10.0. NaCl concentration ranged from 0 to 5% (W/V). The 16S rRNA gene sequence of NEAU-LLCTshowed the high similarities with Microbacterium kyungheense JCM 18735T(98.5%), Microbacterium trichothecenolyticum JCM 1358T(98.3%) and Microbacterium jejuense JCM 18734T(98.2%). The whole-cell sugars were glucose, rhamnose and ribose. The menaquinones contained MK-12 and MK-13. Ornithine, glutamic acid, lysine and a small amount of alanine and glycine were the amino acids in the hydrolyzed products of the cell wall. The major fatty acids were iso-C16:0, iso-C18:0, anteiso-C15:0and anteiso-C17:0. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid. The genome of NEAU-LLCTwas 4,369,375 bp and G + C content is 70.28 mol%. A combination of DNA-DNA hybridization result and some phenotypic characteristics demonstrated that strain NEAU-LLCTcould be distinguished from its closely related strains. Therefore, the strain NEAU-LLCTwas considered to represent a novel species, which was named Microbacterium helvum sp. (Type strain NEAU-LLCT= CCTCC AA 2018026T= JCM 32661T).
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