Gene expression changes: five years after creation of elastase-induced aneurysms.
Gene expression changes: five years after creation of elastase-induced aneurysms.
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DOI：
10.1016/j.jvir.2011.01.447
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发表时间：
2011-10
影响因子：
2.9
通讯作者：
Kallmes, David F.
中科院分区：
文献类型：
--
作者：
Kadirvel, Ramanathan;Ding, Yong-Hong;Dai, Daying;Lewis, Debra A.;Kallmes, David F.
颅内囊状动脉瘤与动脉壁的慢性重塑有关。人们对动脉瘤生长和破裂的病理学知之甚少。本研究的目的是研究动脉瘤形成五年后，与对照未手术动脉相比，弹性蛋白酶诱导的兔子囊状动脉瘤的基因表达模式。在 25 只兔子身上制造了弹性蛋白酶诱导的囊状动脉瘤，并进行了长达五年的随访。 13只兔子在随访期间因与动脉瘤无关的原因死亡。从 12 名幸存受试者中的 5 名受试者的动脉瘤组织和对照对侧颈总动脉中分离出 RNA，并使用人类基因微阵列分析基因表达。组间具有统计差异的基因（p<0.05且倍数变化≥1.5且≤0.75）被认为是差异表达。 RT-PCR 用于确认所选基因的基因微阵列结果。与未手术的对照动脉相比，动脉瘤中 13,353 个基因中有 50 个（0.33%）存在差异表达。分子和功能通路分析显示，与对照组相比，动脉瘤中免疫调节分子、生长因子、细胞粘附分子和结构分子的表达存在差异。所选基因的RT-PCR结果证实了使用基因芯片微阵列鉴定的差异表达。慢性动脉瘤中各种生化和细胞功能的显着调节为囊状动脉瘤的病理生理学提供了分子见解。
Intracranial, saccular aneurysms are associated with chronic remodeling of the arterial wall. The pathobiology of aneurysm growth and rupture is poorly understood. The objective of the present study was to study the gene expression patterns in elastase-induced saccular aneurysms in rabbits five years after aneurysm creation as compared to control, unoperated arteries. Elastase-induced saccular aneurysms were created in 25 rabbits and followed up to five years. Thirteen rabbits died during follow-up for reasons unrelated to the aneurysms. RNA was isolated from aneurysm tissue and the control contralateral common carotid artery in 5 of the 12 surviving subjects and analyzed for gene expression using human gene microarrays. Genes with statistical differences between groups (p<0.05 and fold change ≥1.5 and ≤0.75) were considered differentially expressed. RT-PCR was used for confirmation of gene microarray findings for selected genes. Fifty (0.33%) of 13,353 genes were differentially expressed in the aneurysms compared with the unoperated control arteries. Molecular and functional pathway analysis revealed that immunoregulatory molecules, growth factors, cell adhesion molecules, and structural molecules were differentially expressed in the aneurysms as compared to controls. RT-PCR results of selected genes confirmed the differential expression identified using the gene chip microarray. Significant modulation in a variety of biochemical and cellular functions in chronic aneurysms provides molecular insights into the pathophysiology of saccular aneurysms.
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影响因子：
4.8
作者：
Jayaraman, T;Berenstein, V;Berenstein, A
通讯作者：
Berenstein, A
影响因子：
2.8
作者：
Ding, YH;Dai, DY;Kallmes, DF
通讯作者：
Kallmes, DF
影响因子：
2.4
作者：
RYBA, M;GRIEB, P;GORSKI, A
通讯作者：
GORSKI, A
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
4.8
作者：
Tulamo, Riikka;Frosen, Juhana;Meri, Seppo
通讯作者：
Meri, Seppo