Isolation and characterization of highly pathogenic avian influenza virus subtype H5N1 from donkeys.
Isolation and characterization of highly pathogenic avian influenza virus subtype H5N1 from donkeys.
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DOI：
10.1186/1423-0127-17-25
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发表时间：
2010-04-14
影响因子：
11
通讯作者：
Shany SA
中科院分区：
文献类型：
--
作者：
Abdel-Moneim AS;Abdel-Ghany AE;Shany SA
高致病性H5N1是一种跨越物种障碍的主要禽类病原体，严重影响人类和一些哺乳动物。它以一种加剧的方式发生变异，并被认为是可能发生的下一次大流行的潜在候选者，具有所有灾难性后果。鼻拭子是从患有呼吸窘迫的驴身上采集的。该病毒是从无特定病原体的鸡胚蛋(SPF-ECA)中混合的鼻拭子中分离出来的。逆转录-聚合酶链式反应(RT-PCR)和血凝素、神经氨酸酶测序。用血凝抑制试验(HI)对105份驴血清进行了H5血清转换试验。我们证明了H5N1从家禽传播到另一种哺乳动物宿主--驴。系统进化分析表明，该病毒聚集在来自埃及的H5N1毒株谱系中，与2009年分离的毒株密切相关。与来自禽类和人类的密切相关的病毒相比，它几乎没有遗传变化。神经氨酸酶缺乏对奥司他韦耐药的突变。有趣的是，对驴的H5血凝素抗体进行HI筛查显示，接触率很高。这些发现扩大了H5N1流感病毒的宿主范围，对流感病毒流行病学具有影响，并突出表明需要对后院家禽单位附近的动物，特别是流行地区的动物进行H5N1病毒的系统监测。
The highly pathogenic H5N1 is a major avian pathogen that crosses species barriers and seriously affects humans as well as some mammals. It mutates in an intensified manner and is considered a potential candidate for the possible next pandemic with all the catastrophic consequences. Nasal swabs were collected from donkeys suffered from respiratory distress. The virus was isolated from the pooled nasal swabs in specific pathogen free embryonated chicken eggs (SPF-ECE). Reverse transcriptase polymerase chain reaction (RT-PCR) and sequencing of both haemagglutingin and neuraminidase were performed. H5 seroconversion was screened using haemagglutination inhibition (HI) assay on 105 donkey serum samples. We demonstrated that H5N1 jumped from poultry to another mammalian host; donkeys. Phylogenetic analysis showed that the virus clustered within the lineage of H5N1 from Egypt, closely related to 2009 isolates. It harboured few genetic changes compared to the closely related viruses from avian and humans. The neuraminidase lacks oseltamivir resistant mutations. Interestingly, HI screening for antibodies to H5 haemagglutinins in donkeys revealed high exposure rate. These findings extend the host range of the H5N1 influenza virus, possess implications for influenza virus epidemiology and highlight the need for the systematic surveillance of H5N1 in animals in the vicinity of backyard poultry units especially in endemic areas.
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影响因子：
56.9
作者：
Kuiken, T;Rimmelzwaan, G;Osterhaus, A
通讯作者：
Osterhaus, A
影响因子：
11.8
作者：
Keawcharoen J;Oraveerakul K;Kuiken T;Fouchier RA;Amonsin A;Payungporn S;Noppornpanth S;Wattanodorn S;Theambooniers A;Tantilertcharoen R;Pattanarangsan R;Arya N;Ratanakorn P;Osterhaus DM;Poovorawan Y
通讯作者：
Poovorawan Y
影响因子：
3.7
作者：
Gambaryan, A;Tuzikov, A;Klimov, A
通讯作者：
Klimov, A
DOI：
10.1073/pnas.0511120103
发表时间：
2006-02-21
影响因子：
11.1
作者：
Chen, H;Smith, GJD;Guan, Y
通讯作者：
Guan, Y
影响因子：
5.8
作者：
Kumar, S;Tamura, K;Nei, M
通讯作者：
Nei, M