Unusual sub-genus associations of faecal Prevotella and Bacteroides with specific dietary patterns.
Unusual sub-genus associations of faecal Prevotella and Bacteroides with specific dietary patterns.
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DOI：
10.1186/s40168-016-0202-1
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发表时间：
2016-10-21
期刊：
影响因子：
15.5
通讯作者：
Ercolini D
中科院分区：
文献类型：
--
作者：
De Filippis F;Pellegrini N;Laghi L;Gobbetti M;Ercolini D
饮食在塑造肠道微生物群方面具有公认的效果。许多研究将普雷沃氏菌的增加与高纤维饮食联系起来，而拟杆菌的丰度通常与动物脂肪和富含蛋白质的饮食有关。然而，密切相关的物种和菌株可能拥有不同的基因库;因此，进一步的研究应旨在了解同属的物种是否始终与饮食模式有关，或对饮食变化同样敏感。在这里，我们使用16 S rRNA基因测序数据的寡分型，利用普雷沃菌属和拟杆菌属的粪便样本中的多样性杂食动物和非杂食动物的主题从以前的研究队列。一个很大的异质性被发现在寡型组成。然而，同一属内的不同寡型显示出独特的相关模式与饮食成分和代谢组。我们发现一些寡型普雷沃氏菌与植物性饮食显著相关，但有些与动物性营养素相关，这同样适用于拟杆菌。因此，一个不加区别的协会拟杆菌属与特定的饮食模式可能会导致一个过于简单的愿景，不考虑亚属的多样性和不同的可能的反应，饮食成分。我们证明了普雷沃氏菌和拟杆菌寡型显示出独特的相关模式与饮食成分和代谢组。这些结果证实了目前对饮食依赖性微生物-宿主关系的过度简化，并强调在计划肠道微生物群调节以获得健康益处时必须考虑亚属差异。本文的在线版本（doi：10.1186/s40168-016-0202-1）包含补充材料，可供授权用户使用。
Diet has a recognized effect in shaping gut microbiota. Many studies link an increase in Prevotella to high-fibre diet, while Bacteroides abundance is usually associated with the consumption of animal fat and protein-rich diets. Nevertheless, closely related species and strains may harbour different genetic pools; therefore, further studies should aim to understand whether species of the same genus are consistently linked to dietary patterns or equally responsive to diet variations. Here, we used oligotyping of 16S rRNA gene sequencing data to exploit the diversity within Prevotella and Bacteroides genera in faecal samples of omnivore and non-omnivore subjects from a previously studied cohort. A great heterogeneity was found in oligotype composition. Nevertheless, different oligotypes within the same genus showed distinctive correlation patterns with dietary components and metabolome. We found that some Prevotella oligotypes are significantly associated with the plant-based diet but some are associated with animal-based nutrients, and the same applies to Bacteroides. Therefore, an indiscriminate association of Bacteroidetes genera with specific dietary patterns may lead to an oversimplified vision that does not take into account sub-genus diversity and the different possible responses to dietary components. We demonstrated that Prevotella and Bacteroides oligotypes show distinctive correlation patterns with dietary components and metabolome. These results substantiate a current oversimplification of diet-dependent microbe-host associations and highlighted that sub-genus differences must be taken into account when planning gut microbiota modulation for health benefits The online version of this article (doi:10.1186/s40168-016-0202-1) contains supplementary material, which is available to authorized users.
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影响因子：
64.5
作者：
Greenblum S;Carr R;Borenstein E
通讯作者：
Borenstein E
DOI：
10.1038/ismej.2014.97
发表时间：
2015-01
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1073/pnas.1409644111
发表时间：
2014-07-15
影响因子：
11.1
作者：
Eren, A. Murat;Borisy, Gary G.;Welch, Jessica L. Mark
通讯作者：
Welch, Jessica L. Mark
DOI：
10.1093/bioinformatics/btp636
发表时间：
2010-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Caporaso JG;Bittinger K;Bushman FD;DeSantis TZ;Andersen GL;Knight R
通讯作者：
Knight R
影响因子：
4.4
作者：
Wang, Qiong;Garrity, George M.;Cole, James R.
通讯作者：
Cole, James R.