Intermittent Parathyroid Hormone Alters Gut Microbiota in Ovariectomized Osteoporotic Rats.
Intermittent Parathyroid Hormone Alters Gut Microbiota in Ovariectomized Osteoporotic Rats.
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间歇性甲状旁腺激素改变卵巢切除骨质疏松大鼠的肠道微生物群
DOI：
10.1111/os.13419
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发表时间：
2022-09
影响因子：
2.1
通讯作者：
Xue, Yuan
中科院分区：
文献类型：
--
作者：
Zhou, Jiaming;Wang, Rui;Zhao, Rui;Guo, Xing;Gou, Pengguo;Bai, He;Lei, Ping;Xue, Yuan
探讨间歇性甲状旁腺激素（PTH）对去卵巢（OVX）骨质疏松大鼠肠道微生物群（GM）的影响。30只雌性Sprague-Dawley大鼠分为假手术组、OVX组和PTH治疗组。治疗3个月后，分别取股骨、血清和粪便进行微CT、生化分析和16S rRNA测序。16S rRNA测序，过滤原始reads，去除嵌合体序列，获得干净reads。根据这些clean reads对操作分类单元（otu）进行聚类。通过维恩图分析，找出三组GM的共性和独特性。利用Shannon和Simpson指数进行α‐多样性分析，评价基因丰富度和多样性，利用β‐多样性分析评估基因结构，利用Tax4Fun分析预测基因代谢功能。显微CT和生化分析显示，与OVX组相比，PTH组有显著改善。在Venn图分析中，SHAM和PTH组比OVX组发现更多独特的otu。根据秩丰度曲线，SHAM和PTH组具有相似的丰富度和均匀度，均高于OVX组。与OVX组相比，SHAM和PTH组的Simpson和Shannon指数更高，说明SHAM和PTH组的微生物群复杂性高于OVX组。在β -多样性分析中，OVX组与PTH和SHAM组明显分离，这表明骨质疏松是影响GM组成和PTH治疗的关键因素，可以恢复GM的结构。与OVX组相比，PTH治疗增加了GM的丰度，这些GM可以增加骨量，如乳酸杆菌、Muribaculaceae、Ruminococcaceae和Clostridia。并抑制Rikenellaceae的相对丰度，据报道Rikenellaceae可能与骨质疏松症有关。GM功能分析表明，甲状旁腺素能促进丁酸酯的合成。在Tax4Fun分析中，PTH组的丁酸盐代谢功能比OVX和SHAM组更重要，提示PTH治疗可以调节微生物代谢功能，包括丁酸盐代谢。间断性甲状旁腺激素可通过增加益生菌丰度和降低致病菌丰度与GM相互作用，提高骨量。间歇性甲状旁腺激素可以通过增加肠道益生菌的丰度和减少致病菌的丰度来与肠道微生物群相互作用，从而增加骨量。
To investigate the effect of intermittent parathyroid hormone (PTH) on gut microbiota (GM) in ovariectomized (OVX) osteoporotic rats. Thirty female Sprague–Dawley rats were divided into three groups: sham‐operation (SHAM) group, OVX group and PTH treatment group. After 3 months of treatment, the femurs, serum and feces were acquired for micro‐CT, biochemical analysis and 16S rRNA sequencing, respectively. For 16S rRNA sequencing, after raw reads filtrated and chimera sequences removed, the clean reads were obtained. According to these clean reads, the operational taxonomic units (OTUs) were clustered. Venn diagram analysis was conducted to explore common and unique GM among the three groups. The α‐diversity analysis including Shannon and Simpson indexes were used to evaluate the richness and diversity of the GM. The β‐diversity analysis was performed to estimate the structure of GM. The metabolic function was predicted by Tax4Fun analysis. With micro‐CT and biochemical analysis, significant improvements were found in the PTH group compared with the OVX group. In Venn diagram analysis, more unique OTUs were found in the SHAM and PTH groups than the OVX group. According to the rank abundance curve, the SHAM and PTH groups had similar richness and evenness, which were higher than the OVX group. Simpson and Shannon indexes were higher in the SHAM and PTH groups compared with the OVX group, indicating that the SHAM and PTH groups had higher microbiota complexity than the OVX group. In β‐diversity analysis, apparent separation was found in the OVX group from the PTH and SHAM groups, which suggested that osteoporosis is the critical factor influencing the GM composition and PTH treatment and can restore the structure of GM. Compared with the OVX group, treatment with PTH increased the abundances of GM which were reported to increase bone mass, such as Lactobacillus_reuteri, Muribaculaceae, Ruminococcaceae, and Clostridia, and inhibited the relative abundance of Rikenellaceae, which was reported to be potentially related to osteoporosis. GM function analysis showed that PTH could promote butyrate synthesis. In Tax4Fun analysis, the function of butanoate metabolism is more vital in the PTH group than the OVX and SHAM groups, suggesting PTH treatment could regulate microbial metabolic function, including butanoate metabolism. Intermittent PTH can interact with GM through increasing the abundance of probiotics and reducing the abundance of the pathogenic bacteria to enhance the bone mass. Intermittent parathyroid hormone can interact with the gut microbiota by increasing the abundance of gut probiotics and reducing the abundance of the pathogenic bacteria to enhance the bone mass.
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影响因子：
5.6
作者：
Britton, Robert A.;Irwin, Regina;Quach, Darin;Schaefer, Laura;Zhang, Jing;Lee, Taehyung;Parameswaran, Narayanan;McCabe, Laura R.
通讯作者：
McCabe, Laura R.
影响因子：
15.9
作者：
Li, Jau-Yi;Chassaing, Benoit;Pacifici, Roberto
通讯作者：
Pacifici, Roberto
影响因子：
6.2
作者：
Burge, Russel;Dawson-Hughes, Bess;Tosteson, Anna
通讯作者：
Tosteson, Anna
影响因子：
56.9
作者：
Gill, Steven R.;Pop, Mihai;Nelson, Karen E.
通讯作者：
Nelson, Karen E.
影响因子：
4
作者：
Blume, S. W.;Curtis, J. R.
通讯作者：
Curtis, J. R.