Building programmable multicompartment artificial cells incorporating remotely activated protein channels using microfluidics and acoustic levitation.
Building programmable multicompartment artificial cells incorporating remotely activated protein channels using microfluidics and acoustic levitation.
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DOI：
10.1038/s41467-022-31898-w
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发表时间：
2022-07-15
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
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作者：
细胞内区室是通过化学反应和生物过程的时空调节来支持活细胞内新陈代谢的功能单位。因此，作为自下而上创建人造细胞的一步，构建类似的细胞内结构对于扩展细胞模拟功能至关重要。在此，我们报告了液滴实验室平台的开发，该平台利用微流体和声悬浮来设计复杂的基于乳液的多室人造细胞。这种悬浮模型为化学物质的划分提供了独立的、动态的、可定义的液滴网络。同样，它们可以通过气动、加热和磁性元件进行远程操作，以进行后处理，包括膜蛋白的掺入； α-溶血素；和大电导的机械敏感通道。液滴网络的组装是三维图案化的，流体输入配置决定液滴含量和连接性，同时可以利用声学操纵在原位重新配置液滴网络。通过应用声场和磁场按需调节膜张力，可以在悬浮的人造细胞中重复激活和停用机械敏感通道。这提供了超越一次性化学介导激活的可能性，以提供膜蛋白功能的重复、非接触控制。总的来说，这扩大了我们不断增长的能力，将日益复杂的人造细胞作为栩栩如生的材料进行编程和操作。工程微乳液在自下而上合成细胞的创建中发挥着关键作用。在这里，作者展示了一个液滴实验室平台，利用微流体和声悬浮来控制功能性人造细胞中的膜蛋白门控。
Intracellular compartments are functional units that support the metabolism within living cells, through spatiotemporal regulation of chemical reactions and biological processes. Consequently, as a step forward in the bottom-up creation of artificial cells, building analogous intracellular architectures is essential for the expansion of cell-mimicking functionality. Herein, we report the development of a droplet laboratory platform to engineer complex emulsion-based, multicompartment artificial cells, using microfluidics and acoustic levitation. Such levitated models provide free-standing, dynamic, definable droplet networks for the compartmentalisation of chemical species. Equally, they can be remotely operated with pneumatic, heating, and magnetic elements for post-processing, including the incorporation of membrane proteins; alpha-hemolysin; and mechanosensitive channel of large-conductance. The assembly of droplet networks is three-dimensionally patterned with fluidic input configurations determining droplet contents and connectivity, whilst acoustic manipulation can be harnessed to reconfigure the droplet network in situ. The mechanosensitive channel can be repeatedly activated and deactivated in the levitated artificial cell by the application of acoustic and magnetic fields to modulate membrane tension on demand. This offers possibilities beyond one-time chemically mediated activation to provide repeated, non-contact, control of membrane protein function. Collectively, this expands our growing capability to program and operate increasingly sophisticated artificial cells as life-like materials. Engineering micro-emulsion plays a key role in the creation of bottom-up synthetic cells. Here, authors demonstrate a droplet laboratory platform to control membrane protein gating in functional artificial cell using microfluidics and acoustic levitation.
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影响因子：
17.1
作者：
Cazimoglu I;Booth MJ;Bayley H
通讯作者：
Bayley H
影响因子：
15.1
作者：
Einfalt, Tomaz;Garni, Martina;Palivan, Cornelia G.
通讯作者：
Palivan, Cornelia G.
影响因子：
4
作者：
Fushimi, T.;Hil, T. L.;Drinkwater, B. W.
通讯作者：
Drinkwater, B. W.
影响因子：
15
作者：
Deng, Nan-Nan;Yelleswarapu, Maaruthy;Huck, Wilhelm T. S.
通讯作者：
Huck, Wilhelm T. S.
DOI：
10.1126/science.aat4318
发表时间：
2018-05-11
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hahn A;Vonck J;Mills DJ;Meier T;Kühlbrandt W
通讯作者：
Kühlbrandt W