Microwave measurement of giant unilamellar vesicles in aqueous solution.
Microwave measurement of giant unilamellar vesicles in aqueous solution.
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DOI：
10.1038/s41598-017-18806-9
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发表时间：
2018-01-11
影响因子：
4.6
通讯作者：
Wang P
中科院分区：
文献类型：
--
作者：
Cui Y;Delaney WF;Darroudi T;Wang P
用微波技术测量了25μm宽、18.8μm高的微流控通道中漂浮的巨型单层囊泡(GUV)膜。测量频率为2.7Ghz和7.9Ghz，在此频率下，开环谐振器的工作模式为奇模。使用500 nm宽和100μm长的SRR分裂间隙来扫描直径略大于25μm的GUV。较小的流道导致平坦的GUV膜段，与SRR间隙表面紧密接触。用POPC(16：0~18：1PC)、1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine)，SM(16：0卵鞘磷脂)和不同分子组成的胆固醇合成用过的GUV。结果表明，SM和POPC双层膜具有不同的介电常数，其介电常数也随测量频率的不同而变化。得到的膜的介电常数为73.64-j6.13，比以前报道的结果大10倍以上，频率为2.7千兆赫( )。这种差异可能是由于测量的介电极化平行于膜表面，而不是垂直于膜表面。POPC和富SM的GUV表面也被清楚地识别出来。还需要进一步的工作来验证所获得的大介电常数值，并能够准确地分析膜成分。
A microwave technique is demonstrated to measure floating giant unilamellar vesicle (GUV) membranes in a 25 μm wide and 18.8 μm high microfluidic channel. The measurement is conducted at 2.7 and 7.9 GHz, at which a split-ring resonator (SRR) operates at odd modes. A 500 nm wide and 100 μm long SRR split gap is used to scan GUVs that are slightly larger than 25 μm in diameter. The smaller fluidic channel induces flattened GUV membrane sections, which make close contact with the SRR gap surface. The used GUVs are synthesized with POPC (16:0–18:1 PC 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine), SM (16:0 Egg Sphingomyelin) and cholesterol at different molecular compositions. It is shown that SM and POPC bilayers have different dielectric permittivity values, which also change with measurement frequencies. The obtained membrane permittivity values, e.g. 73.64-j6.13 for POPC at 2.7 GHz, are more than 10 times larger than previously reported results. The discrepancy is likely due to the measurement of dielectric polarization parallel with, other than perpendicular to, the membrane surface. POPC and SM-rich GUV surface sections are also clearly identified. Further work is needed to verify the obtained large permittivity values and enable accurate analysis of membrane composition.
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DOI：
10.1109/tmtt.2014.2366134
发表时间：
2014-12
影响因子：
4.3
作者：
Cui Y;Wang P
通讯作者：
Wang P
影响因子：
4
作者：
Gramse, G.;Edwards, M. A.;Gomila, G.
通讯作者：
Gomila, G.
影响因子：
3.4
作者：
Howland, Michael C.;Szmodis, Alan W.;Parikh, Atul N.
通讯作者：
Parikh, Atul N.
DOI：
10.1016/0302-4598(96)05064-7
发表时间：
1996-08-01
期刊：
BIOELECTROCHEMISTRY AND BIOENERGETICS
影响因子：
--
作者：
Coster, HGL;Chilcott, TC;Coster, ACF
通讯作者：
Coster, ACF
影响因子：
3.4
作者：
Lynch, Brian P.;Hilton, Al M.;Simpson, Garth J.
通讯作者：
Simpson, Garth J.