uORF-mediated translation allows engineered plant disease resistance without fitness costs.
uORF-mediated translation allows engineered plant disease resistance without fitness costs.
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uORF介导的翻译允许工程植物抗病，而无需付出适应成本。
DOI：
10.1038/nature22372
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发表时间：
2017-05-25
期刊：
影响因子：
64.8
通讯作者：
Dong X
中科院分区：
文献类型：
--
作者：
Xu G;Yuan M;Ai C;Liu L;Zhuang E;Karapetyan S;Wang S;Dong X
自农业出现以来，控制植物病害一直是人类的一场斗争。对植物免疫机制的研究已经产生了通过植物自身防御基因（例如主免疫调节基因 NPR1）的异位转录来工程化抗性作物的策略。然而，通过这种策略获得的增强的抗性通常与对健康的严重损害相关，使得所得产品不适合农业应用。为了解决这个问题，我们寻求更严格的表达防御蛋白的机制。基于我们最新的发现，即TBF1等关键免疫调节因子的翻译在病原体攻击后被快速且短暂地诱导（随附手稿），我们开发了“TBF1盒”，该盒不仅包含免疫诱导启动子，还包含TBF1基因的两个病原体响应上游开放阅读框（uORFsTBF1）。我们证明，纳入 uORFsTBF1 介导的对拟南芥 (At) 中 snc1（一种自激活免疫受体）和水稻中 AtNPR1 产生的翻译控制，使我们能够在不影响实验室或田间植物适应性的情况下设计广谱抗病性。这种广泛适用的新策略可能会减少农药的使用并减轻对耐药病原体的选择压力。
Controlling plant disease has been a struggle for mankind since the advent of agriculture. Studies of plant immune mechanisms have led to strategies of engineering resistant crops through ectopic transcription of plants’ own defence genes, such as the master immune regulatory gene NPR1. However, enhanced resistance obtained through such strategies is often associated with significant penalties to fitness, making the resulting products undesirable for agricultural applications. To remedy this problem, we sought more stringent mechanisms of expressing defence proteins. Based on our latest finding that translation of key immune regulators, such as TBF1, is rapidly and transiently induced upon pathogen challenge (accompanying manuscript), we developed “TBF1-cassette” consisting of not only the immune-inducible promoter but also two pathogen-responsive upstream open reading frames (uORFsTBF1) of the TBF1 gene. We demonstrate that inclusion of the uORFsTBF1-mediated translational control over the production of snc1 (an autoactivated immune receptor) in Arabidopsis (At) and AtNPR1 in rice enables us to engineer broad-spectrum disease resistance without compromising plant fitness in the laboratory or in the field. This broadly applicable new strategy may lead to reduced use of pesticides and lightening of selective pressure for resistant pathogens.
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DOI：
10.1126/science.1236011
发表时间：
2013-08-16
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Dangl JL;Horvath DM;Staskawicz BJ
通讯作者：
Staskawicz BJ
影响因子：
3.5
作者：
Li, X;Clarke, JD;Dong, XN
通讯作者：
Dong, XN
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
3.5
作者：
Fitzgerald, HA;Chern, MS;Ronald, PC
通讯作者：
Ronald, PC
影响因子：
4.4
作者：
Lawless C;Pearson RD;Selley JN;Smirnova JB;Grant CM;Ashe MP;Pavitt GD;Hubbard SJ
通讯作者：
Hubbard SJ