Cell surface expression of v-fms-coded glycoproteins is required for transformation
Cell surface expression of v-fms-coded glycoproteins is required for transformation
复制标题
转化需要细胞表面表达 v-fms 编码的糖蛋白
DOI：
--
复制
发表时间：
1984
影响因子：
5.3
通讯作者：
C. Sherr
中科院分区：
文献类型：
--
作者：
M. Roussel;C. W. Rettenmier;A. Look;C. Sherr
The viral oncogene v-fms encodes a transforming glycoprotein with in vitro tyrosine-specific protein kinase activity. Although most v-fms-coded molecules remain internally sequestered in transformed cells, a minor population of molecules is transported to the cell surface. An engineered deletion mutant lacking 348 base pairs of the 3.0-kilobase-pair v-fms gene encoded a polypeptide that was 15 kilodaltons smaller than the wild-type v-fms gene product. The in-frame deletion of 116 amino acids was adjacent to the transmembrane anchor peptide located near the middle of the predicted protein sequence and 432 amino acids from the carboxyl terminus. The mutant polypeptide acquired N-linked oligosaccharide chains, was proteolytically processed in a manner similar to the wild-type glycoprotein, and exhibited an associated tyrosine-specific protein kinase activity in vitro. However, the N-linked oligosaccharides of the mutant glycoprotein were not processed to complex carbohydrate chains, and the glycoprotein was not detected at the cell surface. Cells expressing high levels of the mutant glycoprotein did not undergo morphological transformation and did not form colonies in semisolid medium. The transforming activity of the v-fms gene product therefore appears to be mediated through target molecules on the plasma membrane.
影响因子：
56.9
作者：
DOOLITTLE, RF;HUNKAPILLER, MW;ANTONIADES, HN
通讯作者：
ANTONIADES, HN
小鼠 L 细胞稳定转化为人膜 T 细胞分化抗原、HLA 和 β2-微球蛋白：通过荧光激活细胞分选进行选择。
DOI：
10.1073/pnas.80.2.524
发表时间：
1983
影响因子：
11.1
作者：
Kavathas,P;Herzenberg,LA
通讯作者：
Herzenberg,LA
影响因子：
56.9
作者：
SLAMON, DJ;DEKERNION, JB;CLINE, MJ
通讯作者：
CLINE, MJ