Identification of QTLs associated with oil content in a high-oil Brassica napus cultivar and construction of a high-density consensus map for QTLs comparison in B. napus.
Identification of QTLs associated with oil content in a high-oil Brassica napus cultivar and construction of a high-density consensus map for QTLs comparison in B. napus.
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DOI：
10.1371/journal.pone.0080569
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Wang X;Wang H;Long Y;Li D;Yin Y;Tian J;Chen L;Liu L;Zhao W;Zhao Y;Yu L;Li M
提高种子含油量是油菜育种的重要目标之一。napus L.）。剖析B油分含量的遗传基础。以油分差异大于10%的“垦C-8”与“N53- 2”杂交，获得了一个包含348个株系的大型双单倍体（DH）群体，命名为KN DH群体。构建了一张由403个标记组成的遗传连锁图谱，图谱总长度为1783.9cM，平均标记间距为4.4cM。KN DH群体在8个自然环境中进行表型分析，并进行含油量的数量性状基因座（QTL）分析。共检测到63个QTL，对表型变异的解释率为2.64-17.88%，通过Meta分析，这些QTL进一步整合到位于11条染色体上的24个共有QTL中。将KN DH图谱与其他7个已发表的基于共有标记的图谱相结合，构建了一个包含1335个标记位点的高密度共有图谱。在KN DH群体的24个共有QTL中，有14个为新QTL，其中5个位于A基因组，9个位于C基因组。结果表明，油分含量差异显著的群体越大，检测新的油分含量QTL的把握度越高，一致性图谱的构建为不同群体油分含量QTL的比较提供了新的线索。这些结果丰富了对油分含量QTL的认识，为B的分子标记辅助育种提供了潜在的应用价值。油菜。
Increasing seed oil content is one of the most important goals in breeding of rapeseed (B. napus L.). To dissect the genetic basis of oil content in B. napus, a large and new double haploid (DH) population containing 348 lines was obtained from a cross between ‘KenC-8’ and ‘N53-2’, two varieties with >10% difference in seed oil content, and this population was named the KN DH population. A genetic linkage map consisting of 403 markers was constructed, which covered a total length of 1783.9 cM with an average marker interval of 4.4 cM. The KN DH population was phenotyped in eight natural environments and subjected to quantitative trait loci (QTL) analysis for oil content. A total of 63 identified QTLs explaining 2.64–17.88% of the phenotypic variation were identified, and these QTLs were further integrated into 24 consensus QTLs located on 11 chromosomes using meta-analysis. A high-density consensus map with 1335 marker loci was constructed by combining the KN DH map with seven other published maps based on the common markers. Of the 24 consensus QTLs in the KN DH population, 14 were new QTLs including five new QTLs in A genome and nine in C genome. The analysis revealed that a larger population with significant differences in oil content gave a higher power detecting new QTLs for oil content, and the construction of the consensus map provided a new clue for comparing the QTLs detected in different populations. These findings enriched our knowledge of QTLs for oil content and should be a potential in marker-assisted breeding of B. napus.
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