Microgrooved-surface topography enhances cellular division and proliferation of mouse bone marrow-derived mesenchymal stem cells.
Microgrooved-surface topography enhances cellular division and proliferation of mouse bone marrow-derived mesenchymal stem cells.
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微胶状地面地形增强了小鼠骨髓衍生的间充质干细胞的细胞分裂和增殖。
DOI：
10.1371/journal.pone.0182128
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Rath PC
中科院分区：
文献类型：
--
作者：
Chaudhary JK;Rath PC
间充质干细胞（MSC）的命运在很大程度上取决于各种地形特征和一系列细胞外基质（ECM）成分存在于其小生境。除了维持结构稳定性外，它们还调节细胞形态、分裂、增殖、迁移和分化等。传统的MSC培养主要基于培养皿和培养板的二维光滑表面，不提供类似于体内三维壁龛的地形线索，影响各种细胞过程。因此，我们培养的小鼠骨髓来源的MSC的微槽轴承表面，部分模仿在体内网状龛，研究其形态，多能性因子相关的干细胞，细胞分裂和增殖率的影响。培养后，分别通过显微镜和免疫表型分析，观察MSC的形态学特征，MSC特异性标志物基因表达，如CD 29、CD 44、Sca-1沿着以及HSC特异性标志物CD 34、CD 45、CD 11 b。HSC是另一种类型的骨髓干细胞群，其在包括造血在内的各种功能中与MSC协调地相互作用。此外，进一步分析了间充质干细胞的多能性相关转录因子如Oct 3/4、Sox-2、Nanog和Myc的基因表达，以及分化为脂肪细胞、骨细胞和软骨细胞。我们的研究结果表明，微槽表面培养的间充质干细胞（MMSC）表达更高水平的预期细胞表面和多能性相关标志物，增殖更快（2-3倍），S/G2-M期细胞的百分比更高，因此与标准培养瓶生长的细胞（MSC）相比，作为对照，细胞产量更高。此外，MSC和MMSC均显示出相当大的细胞内脂滴积累、较高的碱性磷酸酶活性和细胞外基质分泌，这分别是脂肪生成、骨生成和软骨生成的特征。
Mesenchymal stem cells’ (MSCs) fate is largely determined by the various topographical features and a range of extracellular matrix (ECM) components present in their niches. Apart from maintaining structural stability, they regulate cell morphology, division, proliferation, migration and differentiation among others. Traditional MSC cultures, which are mainly based on two-dimensional smooth surfaces of culture dishes and plates, do not provide topographical cues similar to in vivo three-dimensional niches, impacting various cellular processes. Therefore, we culture the mouse bone marrow-derived MSCs on microgrooved bearing surface, partially mimicking in vivo reticulated niche, to study its effect on morphology, pluripotency factor-associated stemness, cell division and rate of proliferation. Following culture, morphological features, and MSC-specific marker gene expression, such as CD29, CD44, Sca-1 along with HSC (Haematopoietic stem cell)-specific markers like CD34, CD45, CD11b were evaluated by microscopy and immunophenotyping, respectively. HSC is another type of bone marrow stem cell population, which concertedly interacts with MSC during various functions, including haematopoiesis. In addition, mesenchymal stem cells were further analyzed for gene expression of pluripotency-associated transcription factors such as Oct3/4, Sox-2, Nanog and Myc, as well as differentiated into adipocytes, osteocytes and chondrocytes. Our results show that microgrooved surface-cultured mesenchymal stem cells (MMSCs) expressed higher levels of expected cell surface and pluripotency-associated markers and proliferated more rapidly (2–3×fold) with higher percentage of cells in S/G2-M-phase, consequently giving rise to higher cell yield compared to standard culture flask-grown cells (MSCs), taken as control. Furthermore, both MSCs and MMSCs showed considerable accumulation of intracellular lipid-droplets, higher alkaline phosphatase activity and secretion of extracellular matrix that are characteristics of adipogenesis, osteogenesis and chondrogenesis, respectively.
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影响因子：
5.4
作者：
Bao J;Wu Q;Wang Y;Li Y;Li L;Chen F;Wu X;Xie M;Bu H
通讯作者：
Bu H
影响因子：
4
作者：
Freund, Daniel;Bauer, Nicola;Corbeil, Denis
通讯作者：
Corbeil, Denis
影响因子：
4
作者：
Baddoo, M;Hill, K;Phinney, DG
通讯作者：
Phinney, DG
DOI：
10.1111/j.1365-2184.1970.tb00347.x
发表时间：
1970-01-01
期刊：
CELL AND TISSUE KINETICS
影响因子：
--
作者：
FRIEDENSTEIN, AJ;CHAILAKHJAN, RK;LALYKINA, KS
通讯作者：
LALYKINA, KS
DOI：
10.1007/s11626-007-9041-5
发表时间：
2007-10-01
影响因子：
2.1
作者：
Nadri, Samad;Soleimani, Masoud
通讯作者：
Soleimani, Masoud