Dynamic changes impact the plum pox virus population structure during leaf and bud development.
Dynamic changes impact the plum pox virus population structure during leaf and bud development.
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DOI：
10.1016/j.virol.2020.06.014
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发表时间：
2020-09
期刊：
影响因子：
3.7
通讯作者：
Culver JN
中科院分区：
文献类型：
--
作者：
Tamukong YB;Collum TD;Stone AL;Kappagantu M;Sherman DJ;Rogers EE;Dardick C;Culver JN
李痘病毒（Plum pox virus， PPV）是危害核果生产的世界性疫病。它的木质多年生寄主为病毒在多个生长季节的进化提供了一个动态的环境。为了研究季节性寄主发育对PPV种群结构的影响，研究人员利用核糖体相关病毒基因组（称为翻译组）的下一代测序，评估了李子叶片和芽发育阶段韧皮部或全叶组织衍生的PPV变异。结果表明，PPV翻译组变异在感染水平较低的芽和新生叶组织中比例较高，而感染水平较高的成熟组织中比例较低。额外的变异分析根据种群频率以及韧皮部和整个组织特异性变异确定了不同的群体。综上所述，这些结果表明PPV种群动态受宿主组织类型和发育阶段的影响。
Plum pox virus (PPV) is a worldwide threat to stone fruit production. Its woody perennial hosts provide a dynamic environment for virus evolution over multiple growing seasons. To investigate the impact seasonal host development plays in PPV population structure, next generation sequencing of ribosome associated viral genomes, termed translatome, was used to assess PPV variants derived from phloem or whole leaf tissues over a range of plum leaf and bud developmental stages. Results show that translatome PPV variants occur at proportionately higher levels in bud and newly developing leaf tissues that have low infection levels while more mature tissues with high infection levels display proportionately lower numbers of viral variants. Additional variant analysis identified distinct groups based on population frequency as well as sets of phloem and whole tissue specific variants. Combined, these results indicate PPV population dynamics are impacted by the tissue type and developmental stage of their host.
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作者：
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