A novel estrogen-regulated avian apolipoprotein.
A novel estrogen-regulated avian apolipoprotein.
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DOI：
10.1016/j.biochi.2013.09.005
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发表时间：
2013-12
期刊：
影响因子：
3.9
通讯作者：
Hermann, Marcela
中科院分区：
文献类型：
--
作者：
Nikolay, Birgit;Plieschnig, Julia A.;Subik, Desiree;Schneider, Jeannine D.;Schneider, Wolfgang J.;Hermann, Marcela
为了寻找与鸡脂质代谢有关的未知蛋白，我们从血清脂蛋白部分分离出一种迄今未知的蛋白，其浮力密度≤1.063 g/ml。通过蛋白质微测序和cDNA分子克隆获得的数据确定了一个537 bp的cDNA，编码178个残基的前体分子。通过SDS-PAGE测定，该蛋白的主要循环形式，我们称之为载脂蛋白- vldl - iv (Apo-IV)，其表观Mr约为17 kDa。对蛋鸡不同组织的Northern Blot分析显示，Apo-IV主要在肝脏和小肠表达，与该蛋白参与脂蛋白代谢相一致。为了进一步研究Apo-IV的生物学特性，我们建立了一种针对GST-Apo-IV融合蛋白的抗体，该抗体可以在公鸡血浆中检测到17-kDa蛋白，而在蛋鸡中，它只能在分离的≤1.063 g/ml的密度脂蛋白部分中检测到。有趣的是，公鸡的雌激素治疗导致肝脏和循环中的Apo-IV减少到与成熟母鸡相似的水平。此外，该抗体与鹌鹑血浆中的一个17 kda蛋白发生交叉反应，表明载脂蛋白iv在禽类物种中具有保守性。在寻找哺乳动物载脂蛋白iv的对应物时，将新鸡蛋白的序列与不同哺乳动物载脂蛋白的序列进行比对，发现与ApoC-IV的区域有有限的相似性，并且可能与各种哺乳动物物种的载脂蛋白e相似。这些数据表明Apo-IV是一种新发现的禽类载脂蛋白。载脂蛋白vldl - iv （Apo-IV）是一种新发现的禽类载脂蛋白。Apo-IV表达受雌激素抑制。含有VLDL的Apo-IV颗粒被排除在蛋黄摄取之外。Apo-IV与哺乳动物ApoC-IV具有有限的相似性。
In search for yet uncharacterized proteins involved in lipid metabolism of the chicken, we have isolated a hitherto unknown protein from the serum lipoprotein fraction with a buoyant density of ≤1.063 g/ml. Data obtained by protein microsequencing and molecular cloning of cDNA defined a 537 bp cDNA encoding a precursor molecule of 178 residues. As determined by SDS-PAGE, the major circulating form of the protein, which we designate apolipoprotein-VLDL-IV (Apo-IV), has an apparent Mr of approximately 17 kDa. Northern Blot analysis of different tissues of laying hens revealed Apo-IV expression mainly in the liver and small intestine, compatible with an involvement of the protein in lipoprotein metabolism. To further investigate the biology of Apo-IV, we raised an antibody against a GST-Apo-IV fusion protein, which allowed the detection of the 17-kDa protein in rooster plasma, whereas in laying hens it was detectable only in the isolated ≤1.063 g/ml density lipoprotein fraction. Interestingly, estrogen treatment of roosters caused a reduction of Apo-IV in the liver and in the circulation to levels similar to those in mature hens. Furthermore, the antibody crossreacted with a 17-kDa protein in quail plasma, indicating conservation of Apo-IV in avian species. In search for mammalian counterparts of Apo-IV, alignment of the sequence of the novel chicken protein with those of different mammalian apolipoproteins revealed stretches with limited similarity to regions of ApoC-IV and possibly with ApoE from various mammalian species. These data suggest that Apo-IV is a newly identified avian apolipoprotein. Apo-VLDL-IV (Apo-IV) is a newly identified avian apolipoprotein. Apo-IV expression is suppressed by estrogen. Apo-IV containing VLDL particles are excluded from uptake into yolk. Apo-IV has limited similarity to mammalian ApoC-IV.
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DOI：
10.1016/s0021-9673(01)89486-3
发表时间：
1990-07-20
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
BROWN, EM;DOWER, HJ
通讯作者：
DOWER, HJ
影响因子：
20.1
作者：
HAVEL, RJ;SHORE, VG;BIER, DM
通讯作者：
BIER, DM
影响因子：
5.3
作者：
Kamboh, MI;Aston, CE;Hamman, RF
通讯作者：
Hamman, RF
DOI：
10.1111/j.1432-1033.1989.tb14996.x
发表时间：
1989-09-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
HERMIER, D;FORGEZ, P;CHAPMAN, MJ
通讯作者：
CHAPMAN, MJ
影响因子：
4.8
作者：
Loijens, JC;Anderson, RA
通讯作者：
Anderson, RA