Complete mtDNA genomes of Anopheles darlingi and an approach to anopheline divergence time.
Complete mtDNA genomes of Anopheles darlingi and an approach to anopheline divergence time.
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DOI：
10.1186/1475-2875-9-127
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发表时间：
2010-05-14
期刊：
影响因子：
3
通讯作者：
Conn JE
中科院分区：
文献类型：
--
作者：
Moreno M;Marinotti O;Krzywinski J;Tadei WP;James AA;Achee NL;Conn JE
完整序列的线粒体基因组（mtDNA）的成员的北方和南方基因型的按蚊（Nyssorhynchus）darlingi用于比较研究，以估计的时间，最近的共同祖先的现代按蚊，以评估在这个分类群的分化，并寻求证据的早期物种形成。对来自伯利兹和巴西的蚊子的mtDNA进行了测序，并进行了结构和碱基组成的比较分析。与系统发育信息的最大似然法被用来检测选择的证据和贝叶斯方法被用来日期之间的亚属Nyssorhynchus和其他按蚊亚属的分裂。达氏按蚊分类群内mtDNA序列的比较不能提供足够的分辨率来建立物种内不同的物种形成单位。此外，在mtDNA的任何蛋白质编码基因中没有发现正选择的证据，纯化选择可能是缺乏多样性的基础。贝叶斯分析支持的结论是，Nyssorhynchus和按蚊+Cellia的最近祖先是现存的约9400万年前。达林按蚊线粒体DNA基因组分析并未为该分类群的物种形成提供支持。所测试的按蚊种群之间分歧的估计日期与冈瓦纳西部的地质分裂（95百万年）一致，并为解释新大陆不存在Cellia和非洲-欧亚大陆不存在Nyssorhynchus提供了额外的支持。
The complete sequences of the mitochondrial genomes (mtDNA) of members of the northern and southern genotypes of Anopheles (Nyssorhynchus) darlingi were used for comparative studies to estimate the time to the most recent common ancestor for modern anophelines, to evaluate differentiation within this taxon, and to seek evidence of incipient speciation. The mtDNAs were sequenced from mosquitoes from Belize and Brazil and comparative analyses of structure and base composition, among others, were performed. A maximum likelihood approach linked with phylogenetic information was employed to detect evidence of selection and a Bayesian approach was used to date the split between the subgenus Nyssorhynchus and other Anopheles subgenera. The comparison of mtDNA sequences within the Anopheles darlingi taxon does not provide sufficient resolution to establish different units of speciation within the species. In addition, no evidence of positive selection in any protein-coding gene of the mtDNA was detected, and purifying selection likely is the basis for this lack of diversity. Bayesian analysis supports the conclusion that the most recent ancestor of Nyssorhynchus and Anopheles+Cellia was extant ~94 million years ago. Analyses of mtDNA genomes of Anopheles darlingi do not provide support for speciation in the taxon. The dates estimated for divergence among the anopheline groups tested is in agreement with the geological split of western Gondwana (95 mya), and provides additional support for explaining the absence of Cellia in the New World, and Nyssorhynchus in the Afro-Eurasian continents.
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