Delayed specific IgM antibody responses observed among COVID-19 patients with severe progression.
Delayed specific IgM antibody responses observed among COVID-19 patients with severe progression.
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DOI：
10.1080/22221751.2020.1766382
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发表时间：
2020-12
影响因子：
13.2
通讯作者：
Zhu Y
中科院分区：
文献类型：
--
作者：
Shen L;Wang C;Zhao J;Tang X;Shen Y;Lu M;Ding Z;Huang C;Zhang J;Li S;Lan J;Wong G;Zhu Y
严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）自被确认为COVID-19的病原体以来，在全球范围内迅速传播。该疾病的分子诊断通常通过对来自拭子、痰或支气管肺泡灌洗液（BALF）的病毒进行基于核酸的检测来进行。然而，常用标本（拭子或痰）的阳性率低于75%。需要SARS-CoV-2的免疫测定来准确诊断COVID-19。血清收集自中国湖北省襄阳市当地医院的患者或健康人。采用胶体金免疫层析法（GICA）检测血清中SARS-CoV-2特异性IgM抗体。结果结合血清采集日期和临床资料进行分析。发现GICA阳性，其中检测到82.2%（37/45）的RT-qPCR确诊的COVID-19病例，以及32.0%（8/25）的临床确诊的RT-qPCR阴性患者（症状发作后4-14天）。对IgM阴性、RT-qPCR阳性的COVID-19患者的调查显示，其中一半人发展为严重疾病。GICA被发现是一种有用的测试，可以补充现有的基于PCR的检测方法，用于确认COVID-19，并且在严重进展的COVID-19患者中观察到延迟的特异性IgM抗体应答。
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread rapidly worldwide since it was confirmed as the causative agent of COVID-19. Molecular diagnosis of the disease is typically performed via nucleic acid-based detection of the virus from swabs, sputum or bronchoalveolar lavage fluid (BALF). However, the positive rate from the commonly used specimens (swabs or sputum) was less than 75%. Immunological assays for SARS-CoV-2 are needed to accurately diagnose COVID-19. Sera were collected from patients or healthy people in a local hospital in Xiangyang, Hubei Province, China. The SARS-CoV-2 specific IgM antibodies were then detected using a SARS-CoV-2 IgM colloidal gold immunochromatographic assay (GICA). Results were analysed in combination with sera collection date and clinical information. The GICA was found to be positive with the detected 82.2% (37/45) of RT-qPCR confirmed COVID-19 cases, as well as 32.0% (8/25) of clinically confirmed, RT-qPCR negative patients (4–14 days after symptom onset). Investigation of IgM-negative, RT-qPCR-positive COVID-19 patients showed that half of them developed severe disease. The GICA was found to be a useful test to complement existing PCR-based assays for confirmation of COVID-19, and a delayed specific IgM antibody response was observed among COVID-19 patients with severe progression.
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影响因子：
19.7
作者：
Caruso, Damiano;Zerunian, Marta;Laghi, Andrea
通讯作者：
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影响因子：
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DOI：
10.1073/pnas.0409065102
发表时间：
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影响因子：
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影响因子：
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