Functional characterization in Caenorhabditis elegans of transmembrane worm-human orthologs.
Functional characterization in Caenorhabditis elegans of transmembrane worm-human orthologs.
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DOI：
10.1186/1471-2164-5-85
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发表时间：
2004-11-08
期刊：
影响因子：
4.4
通讯作者：
Gomes AV
中科院分区：
文献类型：
--
作者：
Henricson A;Sonnhammer EL;Baillie DL;Gomes AV
完整的基因组序列为人类和线虫秀丽隐杆线虫提供了一个机会，了解更多的人类基因功能，通过功能特性的直系同源物在蠕虫。基于先前对蠕虫-人正向跨膜蛋白的全基因组分析，我们选择了17个基因在C.优雅的选择这些基因的基础是它们都具有高置信度的候选人直系同源物，并且它们的功能未知。我们首先分析了它们的发生、膜拓扑结构和结构域组织。利用RNA干扰技术和转录型gfp报告基因融合技术对该虫基因功能进行了实验研究。这些实验为所研究的12个基因提供了功能性见解。例如，C36B1.12，三个早老素样基因的蠕虫直系同源物，几乎只在头部神经元中表达，这表明一个古老的保守作用对神经元功能很重要。我们提出了一个新的跨膜拓扑结构的早老素样蛋白家族。sft-4是Surf-4基因的同源基因，是虫体发育所必需的基因。R155.1，其人类直系同源物是完全未知的，涉及身体大小控制和其他发育过程。将生物信息学和C. elegans的直系同源物的实验，我们提供了十二个以前未表征的人类基因的功能见解。
The complete genome sequences for human and the nematode Caenorhabditis elegans offer an opportunity to learn more about human gene function through functional characterization of orthologs in the worm. Based on a previous genome-wide analysis of worm-human orthologous transmembrane proteins, we selected seventeen genes to explore experimentally in C. elegans. These genes were selected on the basis that they all have high confidence candidate human orthologs and that their function is unknown. We first analyzed their phylogeny, membrane topology and domain organization. Then gene functions were studied experimentally in the worm by using RNA interference and transcriptional gfp reporter gene fusions. The experiments gave functional insights for twelve of the genes studied. For example, C36B1.12, the worm ortholog of three presenilin-like genes, was almost exclusively expressed in head neurons, suggesting an ancient conserved role important to neuronal function. We propose a new transmembrane topology for the presenilin-like protein family. sft-4, the worm ortholog of surfeit locus gene Surf-4, proved to be an essential gene required for development during the larval stages of the worm. R155.1, whose human ortholog is entirely uncharacterized, was implicated in body size control and other developmental processes. By combining bioinformatics and C. elegans experiments on orthologs, we provide functional insights on twelve previously uncharacterized human genes.
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影响因子：
2.9
作者：
JONES, DT;TAYLOR, WR;THORTON, JM
通讯作者：
THORTON, JM
影响因子：
4.4
作者：
Lorenzi, MV;Castagnino, P;Miki, T
通讯作者：
Miki, T
影响因子：
56.9
作者：
Feinberg, EH;Hunter, CP
通讯作者：
Hunter, CP
DOI：
10.2307/2412448
发表时间：
1970-01-01
期刊：
SYSTEMATIC ZOOLOGY
影响因子：
--
作者：
FITCH, WM
通讯作者：
FITCH, WM
影响因子：
11.6
作者：
Lasswell, J;Rogg, LE;Bartel, B
通讯作者：
Bartel, B