Assessment of clinical sepsis-associated biomarkers in a septic mouse model.
Assessment of clinical sepsis-associated biomarkers in a septic mouse model.
复制标题
脓毒症小鼠模型中临床脓毒症相关生物标志物的评估
DOI：
10.1177/0300060518764717
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发表时间：
2018-06
期刊：
影响因子：
--
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Li JL;Li G;Jing XZ;Li YF;Ye QY;Jia HH;Liu SH;Li XJ;Li H;Huang R;Zhang Y;Wang H
目的利用盲肠结扎穿孔（CLP）脓毒症小鼠模型研究脓毒症的临床相关生物标志物，为探讨脓毒症的病理生理机制和评价新的治疗措施提供参考。方法采用CLP诱导小鼠脓毒症模型，检测小鼠存活率、血液生理生化指标、细胞因子、肝肾功能指标、凝血功能等临床指标。结果死亡率> 70%。体温、血压和心率在48 h内下降。8h时乳酸含量较低。CLP小鼠表现出典型的炎症症状，白色血细胞和降钙素原减少，髓样细胞上表达的可溶性触发受体-1、白细胞介素（IL）-6、IL-10、肿瘤坏死因子-α、巨噬细胞炎性蛋白（MIP）-1α、MIP-1β和MIP-2水平升高。血小板计数和活化部分凝血活酶时间显著降低，凝血酶原时间和凝血酶原时间国际标准化比值显著升高。在CLP模型中发现多器官功能障碍的表型，包括肝丙氨酸转氨酶和天冬氨酸转氨酶升高;总蛋白、球蛋白和血清白蛋白显著降低;血尿素氮和肌酐升高;血糖降低。结论CLP小鼠模型的临床特征与人类脓毒症患者相似。
Objective Clinical sepsis-associated biomarkers were utilized in a cecal ligation and puncture (CLP) septic mouse model to provide a reference for investigating pathophysiological mechanisms and evaluating novel therapeutic interventions for sepsis. Methods Sepsis in mice was induced by CLP, and clinical biomarkers were evaluated (survival rate, blood physiological and biochemical indices, cytokines, hepatorenal function parameters, and blood coagulation). Results The mortality rate was >70%. The body temperature, blood pressure, and heart rate decreased within 48 h. Low lactic acid was found at 8 h. The CLP mice showed typical inflammatory symptoms with decreased white blood cells and procalcitonin and increased levels of soluble triggering receptor expressed on myeloid cells-1, interleukin (IL)-6, IL-10, tumor necrosis factor-α, macrophage inflammatory protein (MIP)-1α, MIP-1β, and MIP-2. The platelet count and activated partial thromboplastin time significantly decreased, and the prothrombin time and prothrombin time–international normalized ratio markedly increased. Phenotypes of multiple organ dysfunction were found in the CLP model, including increased liver alanine aminotransferase and aspartate transaminase; significantly reduced total protein, globulin, and serum albumin; increased blood urea nitrogen and creatinine; and decreased blood glucose. Conclusion The clinical features of the CLP mouse model were similar to those of human patients with sepsis.
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2.2
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