Comparative analysis of fatty acid metabolism based on transcriptome sequencing of wild and cultivated Ophiocordyceps sinensis.
Comparative analysis of fatty acid metabolism based on transcriptome sequencing of wild and cultivated Ophiocordyceps sinensis.
复制标题
基于转录组测序的野生与栽培冬虫夏草脂肪酸代谢比较分析
作者：
Zhang H;Yue P;Tong X;Gao T;Peng T;Guo J
研究背景中华眼镜蛇（Ophiocorphisensis）是青藏高原高寒、高海拔地区的特有种。虽然O. sinensis的栽培，无论是栽培O. sinensis完全可以替代野生O. sinensis还有待确定。方法探讨O.在不同环境下生长的中华绒螯蟹中，我们对具有相同遗传背景的野生和栽培样品进行了形态学和转录组学的比较。结果野生与栽培的野生野生O.中华绒螯蟹的生长环境不同，导致了不同的生长环境。然后，使用Illumina配对末端测序鉴定了总共9，360个转录本。差异表达分析显示，73.89%的差异表达基因（DEG）在O.在自然条件下生长的与在人工条件下生长的比较。功能富集分析表明，野生O.中国产。此外，气相色谱-质谱联用分析结果证实，野生O。中华绒螯蟹（O. sinensis）的总蛋白含量显著高于栽培O.而不饱和脂肪酸所占比例较大。结论这些结果为野生和栽培O.提高人工繁育水平。
Background Ophiocordyceps sinensis is a species endemic to the alpine and high-altitude areas of the Qinghai-Tibet plateau. Although O. sinensis has been cultivated since the past few years, whether cultivated O. sinensis can completely replace wild O. sinensis remains to be determined. Methods To explore the differences of O. sinensis grown in varied environments, we conducted morphological and transcriptomic comparisons between wild and cultivated samples who with the same genetic background. Results The results of morphological anatomy showed that there were significant differences between wild and cultivated O. sinensis, which were caused by different growth environments. Then, a total of 9,360 transcripts were identified using Illumina paired-end sequencing. Differential expression analysis revealed that 73.89% differentially expressed genes (DEGs) were upregulated in O. sinensis grown under natural conditions compared with that grown under artificial conditions. Functional enrichment analysis showed that some key DEGs related to fatty acid metabolism, including acyl-CoA dehydrogenase, enoyl-CoA hydratase, 3-ketoacyl-CoA thiolase, and acetyl-CoA acetyltransferase, were upregulated in wild O. sinensis. Furthermore, gas chromatography-mass spectrometry results confirmed that the fatty acid content of wild O. sinensis was significantly higher than that of cultivated O. sinensis and that unsaturated fatty acids accounted for a larger proportion. Conclusion These results provide a theoretical insight to the molecular regulation mechanism that causes differences between wild and cultivated O. sinensis and improving artificial breeding.
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