Clonal expansion within pneumococcal serotype 6C after use of seven-valent vaccine.
Clonal expansion within pneumococcal serotype 6C after use of seven-valent vaccine.
复制标题
DOI：
10.1371/journal.pone.0064731
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Clarke SC
中科院分区：
文献类型：
--
作者：
Loman NJ;Gladstone RA;Constantinidou C;Tocheva AS;Jefferies JM;Faust SN;O'Connor L;Chan J;Pallen MJ;Clarke SC
Streptococcus pneumoniae causes invasive infections, primarily at the extremes of life. A seven-valent conjugate vaccine (PCV7) is used to protect against invasive pneumococcal disease in children. Within three years of PCV7 introduction, we observed a fourfold increase in serotype 6C carriage, predominantly due to a single clone. We determined the whole-genome sequences of nineteen S. pneumoniae serotype 6C isolates, from both carriage (n = 15) and disease (n = 4) states, to investigate the emergence of serotype 6C in our population, focusing on a single multi-locus sequence type (MLST) clonal complex 395 (CC395). A phylogenetic network was constructed to identify different lineages, followed by analysis of variability in gene sets and sequences. Serotype 6C isolates from this single geographical site fell into four broad phylogenetically distinct lineages. Variation was seen in the 6C capsular locus and in sequences of genes encoding surface proteins. The largest clonal complex was characterised by the presence of lantibiotic synthesis locus. In our population, the 6C capsular locus has been introduced into multiple lineages by independent capsular switching events. However, rapid clonal expansion has occurred within a single MLST clonal complex. Worryingly, plasticity exists within current and potential vaccine-associated loci, a consideration for future vaccine use, target selection and design.
登录
查看更多内容
影响因子：
4.2
作者：
Hussain, M;Melegaro, A;Miller, E
通讯作者：
Miller, E
影响因子：
6.4
作者：
GRAY, BM;CONVERSE, GM;DILLON, HC
通讯作者：
DILLON, HC
影响因子：
9.4
作者：
Jacobs, Michael R.;Bajaksouzian, Saralee;Sampath, Rangarajan
通讯作者：
Sampath, Rangarajan
DOI：
10.1016/j.diagmicrobio.2009.06.020
发表时间：
2009-10-01
影响因子：
2.9
作者：
Campos, Leila C.;Carvalho, Maria da Gloria S.;Reis, Joice N.
通讯作者：
Reis, Joice N.
影响因子：
4.4
作者：
ENGELKE, G;GUTOWSKIECKEL, Z;ENTIAN, KD
通讯作者：
ENTIAN, KD