Overexpression of LiDXS and LiDXR From Lily (Lilium 'Siberia') Enhances the Terpenoid Content in Tobacco Flowers.
Overexpression of LiDXS and LiDXR From Lily (Lilium 'Siberia') Enhances the Terpenoid Content in Tobacco Flowers.
复制标题
百合（百合 – 西伯利亚 –）中 LiDXS 和 LiDXR 的过度表达可提高烟草花中的萜类化合物含量
DOI：
10.3389/fpls.2018.00909
复制
发表时间：
2018
影响因子：
5.6
通讯作者：
Ahmad S
中科院分区：
文献类型：
--
作者：
Zhang T;Sun M;Guo Y;Shi X;Yang Y;Chen J;Zheng T;Han Y;Bao F;Ahmad S
百合是世界著名的切花花卉，其挥发性有机物种类繁多，主要成分为月桂烯、（E）-β-罗勒烯、芳樟醇等单萜类化合物。为了解百合单萜合成的分子机制，我们采用同源PCR方法从香味浓郁的东方百合“西伯利亚”中克隆了2个潜在的4-磷酸甲基化途径基因LiDXS和LiDXR。LiDXS和LiDXR基因的表达水平与单萜类化合物在不同花器官和花发育过程中的释放和积累相一致，表明这两个基因可能在单萜类化合物的合成中起关键作用。亚细胞定位表明LiDXS和LiDXR在叶绿体中表达。转基因烟草中的异位表达表明，与用空载体转化的植物相比，LiDXS和LiDXR转基因系的花积累了更多的二萜、香紫苏醇。令人惊讶的是，在LiDXR转基因株系中检测到单萜、芳樟醇和倍半萜、香紫苏烯的含量增加，而在LiDXS转基因烟草株系中的一个中香紫苏烯的排放增加，表明这两个基因在转基因植物中花挥发物的合成中起重要作用。这些结果表明LiDXR可以促进西伯利亚百合单萜类化合物的生物合成，但LiDXS在单萜类化合物生物合成中的作用有待进一步研究。
Lilium, the famous and significant cut flower, emits a variety of volatile organic compounds, which mainly contain monoterpenes, such as myrcene, (E)-β-ocimene, and linalool. To understand the molecular mechanism of monoterpene synthesis in Lilium, we cloned two potential genes in the methylerythritol 4-phosphate pathway, namely LiDXS and LiDXR, from the strong-flavored oriental Lilium ‘Siberia’ using a homology-based PCR strategy. The expression levels of LiDXS and LiDXR were consistent with the emission and accumulation of monoterpenes in different floral organs and during the floral development, indicating that these two genes may play key roles in monoterpene synthesis. Subcellular localization demonstrated that LiDXS and LiDXR are expressed in the chloroplasts. Ectopic expression in transgenic tobacco suggested that the flowers of LiDXS and LiDXR transgenic lines accumulated substantially more diterpene, sclareol, compared to the plants transformed with empty vector. Surprisingly, increased content of the monoterpene, linalool and sesquiterpene, caryophyllene, were detected in the LiDXR transgenic lines, whereas the emission of caryophyllene, increased in one of the LiDXS transgenic tobacco lines, indicating that these two genes play significant roles in the synthesis of floral volatiles in the transgenic plants. These results demonstrate that LiDXR can contribute to monoterpene biosynthesis in Lilium ‘Siberia’; however, the role of LiDXS in the biosynthesis of monoterpenes needs further study.
登录
查看更多内容
影响因子：
5.6
作者：
Hu Z;Tang B;Wu Q;Zheng J;Leng P;Zhang K
通讯作者：
Zhang K
影响因子：
5.1
作者：
Hsieh, Ming-Hsiun;Chang, Chiung-Yun;Chen, Ju-Jiun
通讯作者：
Chen, Ju-Jiun
影响因子：
7.2
作者：
Burlat, V;Oudin, A;St-Pierre, B
通讯作者：
St-Pierre, B
影响因子：
7.4
作者：
Carretero-Paulet, L;Ahumada, I;Campos, N
通讯作者：
Campos, N
影响因子：
7.2
作者：
Mandel, MA;Feldmann, KA;Leon, P
通讯作者：
Leon, P