Pyramidal and stellate cell specificity of grid and border representations in layer 2 of medial entorhinal cortex.
Pyramidal and stellate cell specificity of grid and border representations in layer 2 of medial entorhinal cortex.
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DOI：
10.1016/j.neuron.2014.11.009
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发表时间：
2014-12-17
期刊：
影响因子：
16.2
通讯作者：
Brecht, Michael
中科院分区：
文献类型：
--
作者：
Tang, Qiusong;Burgalossi, Andrea;Ebbesen, Christian Laut;Ray, Saikat;Naumann, Robert;Schmidt, Helene;Spicher, Dominik;Brecht, Michael
在内侧内嗅皮层，第2层主细胞分为锥体神经元（大多数为钙结合蛋白阳性）和齿状回投射星状细胞（大多数为钙结合蛋白阴性）。我们在自由活动的动物中对第2层神经元进行了细胞标记，但样本量小，无法建立明确的结构-功能关系。然而，我们发现，尖峰锁定θ振荡允许分配身份不明的细胞外记录锥体细胞和星状细胞的特异性分别为83%和89%。在合并的解剖学确定和θ锁定分配的记录，非空间放电占主导地位，弱六边形空间放电和头部方向的选择性，观察到在这两种细胞类型。清晰的网格放电是罕见的，大多数被归类为金字塔（19%，19/99推定的金字塔与3%，3/94推定的星形）。大多数边缘细胞被归类为星状（11%，10/94假定星状vs 1%，1/99假定金字塔）。我们的数据表明，弱theta锁定的星状缘细胞提供空间输入齿状回，而强烈theta锁定的网格放电主要发生在六角形排列的锥体细胞补丁，不饲料到齿状回。星状细胞和锥体细胞可以通过尖峰锁定而被归属于θ细胞，内嗅层2中的网格细胞在很大程度上对应于假定的锥体细胞，第2层中的边界反应在很大程度上对应于假定的星状细胞。研究了内侧内嗅皮层第2层空间表征的细胞基础，并报道了空间放电的细胞类型特异性：大多数网格细胞是假定的锥体神经元，而边缘细胞主要是假定的星状细胞。
In medial entorhinal cortex, layer 2 principal cells divide into pyramidal neurons (mostly calbindin positive) and dentate gyrus-projecting stellate cells (mostly calbindin negative). We juxtacellularly labeled layer 2 neurons in freely moving animals, but small sample size prevented establishing unequivocal structure-function relationships. We show, however, that spike locking to theta oscillations allows assigning unidentified extracellular recordings to pyramidal and stellate cells with ∼83% and ∼89% specificity, respectively. In pooled anatomically identified and theta-locking-assigned recordings, nonspatial discharges dominated, and weakly hexagonal spatial discharges and head-direction selectivity were observed in both cell types. Clear grid discharges were rare and mostly classified as pyramids (19%, 19/99 putative pyramids versus 3%, 3/94 putative stellates). Most border cells were classified as stellate (11%, 10/94 putative stellates versus 1%, 1/99 putative pyramids). Our data suggest weakly theta-locked stellate border cells provide spatial input to dentate gyrus, whereas strongly theta-locked grid discharges occur mainly in hexagonally arranged pyramidal cell patches and do not feed into dentate gyrus. Stellates and pyramids can be putatively assigned by spike locking to theta Grid cells in entorhinal layer 2 largely correspond to putative pyramidal cells Border responses in layer 2 largely correspond to putative stellate cells Tang et al. investigated the cellular basis of spatial representations in layer 2 of medial entorhinal cortex and reported a cell-type specificity of spatial discharges: while most grid cells were putative pyramidal neurons, border cells were mainly putative stellate cells.
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影响因子：
16.2
作者：
Bjerknes, Tale L.;Moser, Edvard I.;Moser, May-Britt
通讯作者：
Moser, May-Britt
DOI：
10.1523/jneurosci.1319-09.2009
发表时间：
2009-08-05
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Lever C;Burton S;Jeewajee A;O'Keefe J;Burgess N
通讯作者：
Burgess N
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
2.5
作者：
Klink, R;Alonso, A
通讯作者：
Alonso, A
DOI：
10.1073/pnas.1209918109
发表时间：
2012-10-23
影响因子：
11.1
作者：
Barry, Caswell;Ginzberg, Lin Lin;Burgess, Neil
通讯作者：
Burgess, Neil