A Perspective on the Use of Fluorescent Imaging to Reveal Mechanisms of Breakup
A Perspective on the Use of Fluorescent Imaging to Reveal Mechanisms of Breakup
复制标题
使用荧光成像揭示破碎机制的观点
DOI：
10.1080/02713683.2022.2098981
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发表时间：
2022
影响因子：
2
通讯作者：
Braun, Richard J.
中科院分区：
文献类型：
--
作者：
King-Smith, Peter Ewen;Begley, Carolyn;Braun, Richard J.
目的：泪膜不稳定性可导致泪膜快速破裂（TBU），被认为是干眼的主要病因。然而，许多TBU机制的理论建议的实验支持是相对稀缺的。这种观点的主要目的是表明，TBU的荧光研究可以用来提供实验证据，提出两个潜在的机制TBU，蒸发和发散flow.Methods：为了了解TBU对泪膜荧光的影响，我们表明，当地的荧光是产品的三个主要因素：泪膜厚度，荧光素浓度和荧光效率。在发散的泪液流中，泪膜厚度减小而荧光素浓度和荧光效率没有变化，从而导致荧光强度减小。蒸发主要通过高荧光素浓度引起的自猝灭引起荧光降低。在高荧光素浓度下淬灭会降低荧光效率，但对于非常低的荧光素浓度，荧光效率与浓度无关;因此，高浓度和非常低浓度的荧光素的比较可用于区分发散流和蒸发。最后，它示出了如何荧光图案可以改变很大的发展过程中breaking.Conclusions：这项分析表明，泪膜荧光TBU和干眼的研究可能是未充分利用的额外信息相关的临床实践中可能会获得。更好地了解TBU机制可能会导致改善干眼的诊断和治疗。
Purpose:Tear film instability, which can lead to rapid tear film breakup (TBU), is considered to be a major etiological factor in dry eye. However, experimental support for many of the proposed theories for TBU mechanisms is relatively scarce. The major aim of this perspective is to show that fluorescence studies of TBU can be used to provide experimental evidence for two proposed underlying mechanisms of TBU, evaporation and divergent flow.Methods:To understand the effects of TBU on tear film fluorescence, we show that local fluorescence is the product of three main factors: tear film thickness, fluorescein concentration and fluorescent efficiency. In divergent tear flow, tear film thickness is reduced without change in fluorescein concentration and fluorescent efficiency, thus leading to reduced fluorescence intensity. Evaporation causes decreased fluorescence mainly by self-quenching due to high fluorescein concentration. Fluorescent efficiency is reduced by quenching at high fluorescein concentration but is independent of concentration for very low fluorescein concentration; thus, comparison of high and very low concentrations of fluorescein can be used to discriminate between divergent flow and evaporation. Finally, it is shown how the fluorescent pattern can change greatly during the development of breakup.Conclusions:This analysis demonstrates that the study of tear film fluorescence in TBU and dry eye may be underutilized as additional information pertinent to clinical practice may be obtained. A better understanding of TBU mechanisms may lead to improved diagnosis and treatment of dry eye.
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作者：
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影响因子：
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DOI：
--
发表时间：
2014
期刊：
影响因子：
--
作者：
Cheng;C. Cerretani;Yuzhang Li;S. Bowers;S. Shahsavarani;M. Lin;C. Radke
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影响因子：
4.4
作者：
King-Smith, P. Ewen;Reuter, Kathleen S.;Nichols, Kelly K.
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Nichols, Kelly K.