The kinetics of chemotactic peptide-induced change in F-actin content, F-actin distribution, and the shape of neutrophils.
The kinetics of chemotactic peptide-induced change in F-actin content, F-actin distribution, and the shape of neutrophils.
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趋化肽诱导的F-肌动蛋白含量，F-肌动蛋白分布和中性粒细胞形状的动力学的动力学。
DOI：
10.1083/jcb.101.3.1078
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发表时间：
1985-09
影响因子：
7.8
通讯作者：
ORESAJO, CO
中科院分区：
文献类型：
--
作者：
HOWARD, TH;ORESAJO, CO
甲酰-甲基亮氨酸(FMLP)诱导中性粒细胞肌动蛋白组装；当fMLP浓度小于或等于10(-8)M时，F-肌动蛋白含量的增加与细胞运动速度的增加相关(Howard，T.H.，and W.H.Meyer，1984，J.Cell Biol.，98：1265-1271)。我们研究了fMLP刺激后F-肌动蛋白含量、F-肌动蛋白分布和细胞形态变化的时程。F-肌动蛋白的含量通过荧光激活的细胞分选分析来定量(Howard，T.H.，1982，J.Cell Biol，95(2，Pt.)2：327A)。通过对硝基苯并恶二唑-菲拉西丁染色细胞的荧光显微照片分析，确定F-肌动蛋白的分布和细胞形态。在25℃的fMLP刺激下，肌动蛋白发生快速的聚合，在45℃的S时达到最大(最高可达对照水平的2.0倍)；随后，刺激5-10分钟后，F-肌动蛋白解聚为中等含量的F-肌动蛋白。F-肌动蛋白的解聚反映了F-肌动蛋白含量的真正下降，因为从细胞中提取的探针量在45 S到10分钟之间也减少了。10(-10)-10(-6)MfMLP的肌动蛋白聚合速率(F-肌动蛋白/S增加3.8+/-0.3-4.4+/-0.6%)与FMLP剂量成反比。在45分钟、S时间和10分钟时，刺激细胞的F-肌动蛋白含量均高于对照组水平，且与fMLP剂量成正比。F-肌动蛋白的分布和细胞形态在刺激后也会随时间变化。45 S刺激后细胞变圆，F-肌动蛋白弥漫分布；刺激10min后细胞极化，F-肌动蛋白局灶性分布。这些结果表明，肌动蛋白的聚合和解聚遵循fMLP刺激的顺序，解聚速度和最大和稳定的F-肌动蛋白含量而不是聚合速度与fMLP剂量相关，在F-肌动蛋白解聚的同时，F-肌动蛋白重新分布，细胞形态发生变化。
Formyl-met-leu-phe (fMLP) induces actin assembly in neutrophils; the resultant increase in F-actin content correlates with an increase in the rate of cellular locomotion at fMLP concentrations less than or equal to 10(-8) M (Howard, T.H., and W.H. Meyer, 1984, J. Cell Biol., 98:1265-1271). We studied the time course of change in F-actin content, F-actin distribution, and cell shape after fMLP stimulation. F-actin content was quantified by fluorescence activated cell sorter analysis of nitrobenzoxadiazole-phallacidin-stained cells (Howard, T.H., 1982, J. Cell Biol., 95(2, Pt. 2:327a). F-actin distribution and cell shape were determined by analysis of fluorescence photomicrographs of nitrobenzoxadiazole-phallacidin-stained cells. After fMLP stimulation at 25 degrees C, there is a rapid actin polymerization that is maximal (up to 2.0 times the control level) at 45 s; subsequently, the F-actin depolymerizes to an intermediate F-actin content 5-10 min after stimulation. The depolymerization of F-actin reflects a true decrease in F-actin content since the quantity of probe extractable from cells also decreases between 45 s and 10 min. The rate of actin polymerization (3.8 +/- 0.3-4.4 +/- 0.6% increase in F-actin/s) is the same for 10(-10) - 10(-6) M fMLP and the polymerization is inhibited by cytochalasin D. The initial rate of F-actin depolymerization (6.0 +/- 1.0-30 +/- 5% decrease in F-actin/min) is inversely proportional to fMLP dose. The F-actin content of stimulated cells at 45 s and 10 min is greater than control levels and varies directly with fMLP dose. F- actin distribution and cell shape also vary as a function of time after stimulation. 45 s after stimulation the cells are rounded and F-actin is diffusely distributed; 10 min after stimulation the cell is polarized and F-actin is focally distributed. These results indicate that actin polymerization and depolymerization follow fMLP stimulation in sequence, the rate of depolymerization and the maximum and steady state F-actin content but not the rate of polymerization are fMLP dose dependent, and concurrent with F-actin depolymerization, F-actin is redistributed and the cell changes shape.
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DOI：
10.1083/jcb.91.3.901
发表时间：
1981-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Yin HL;Albrecht JH;Fattoum A
通讯作者：
Fattoum A
影响因子：
--
作者：
KELLER, HU
通讯作者：
KELLER, HU
影响因子：
15.9
作者：
SELIGMANN, B;CHUSED, TM;GALLIN, JI
通讯作者：
GALLIN, JI
影响因子：
64.8
作者：
SOUTHWICK, FS;HARTWIG, JH
通讯作者：
HARTWIG, JH
影响因子：
7.8
作者：
STOSSEL, TP
通讯作者：
STOSSEL, TP