Collagen fiber crimping following in vivo UVA-induced corneal crosslinking.
Collagen fiber crimping following in vivo UVA-induced corneal crosslinking.
复制标题
DOI：
10.1016/j.exer.2018.08.009
复制
发表时间：
2018-12
影响因子：
3.4
通讯作者：
Jester JV
中科院分区：
文献类型：
--
作者：
Bradford SM;Mikula ER;Juhasz T;Brown DJ;Jester JV
本研究的目的是测量胶原纤维卷曲（CFC）使用二次谐波产生（SHG）信号的非线性光学成像，以确定UVA-核黄素诱导的角膜胶原交联（UVA CXL）对胶原结构的影响。两组，每组四只兔子，右眼用标准UVA CXL治疗。第一组在治疗后1、2和4周进行体内共焦显微镜检查，第二组在治疗后3个月进行共焦显微镜检查，以测量恢复期间的上皮/基质厚度和角膜混浊。分别在一个月和三个月时处死兔子，并在压力下固定它们的角膜。通过胶原自发荧光（CAF）的存在来鉴定交联区域，然后使用SHG显微镜对胶原结构进行成像。CFC的程度通过测量胶原纤维的长度与行进的线性距离之间的百分比差来确定。在中央前部和后部CXL区域、同一角膜的外周非交联区域以及非交联对侧眼的中央角膜中测量CFC。1个月后未检测到角膜厚度变化，但基质厚度在3个月时超过其原始基线厚度25.9 μm。角膜混浊在一个月时达到峰值，然后开始清除。在所有CXL角膜中均检测到CAF增加，在1个月和3个月时，CAF局限于前基质，并延伸至角膜厚度的42.4 ± 3.4%和47.7 ± 7.6%。在所有眼睛中，CAF区域中的CFC在1个月和3个月样本中平均为1.007 ± 0.006和1.009 ± 0.005，而对照组为1.017 ± 0.04和1.016 ± 0.06，有显著降低（P < 0.05）。这些结果表明，UVA CXL后胶原卷曲显著减少约1%。卷曲损失的一种可能解释是CXL区域上的胶原纤维缩短。
The purpose of this study was to measure collagen fiber crimping (CFC) using nonlinear optical imaging of second harmonic generated (SHG) signals to determine the effects of UVA-riboflavin induced corneal collagen crosslinking (UVA CXL) on collagen structure. Two groups, four rabbits each, were treated in the right eye with standard UVA CXL. In vivo confocal microscopy was performed at 1, 2, and 4 weeks after treatment for the first group and up to three months for the second group to measure epithelial/stromal thickness and corneal haze during recovery. Rabbits were sacrificed at one and three months, respectively, and their corneas fixed under pressure. Regions of crosslinking were identified by the presence of collagen autofluorescence (CAF) and then collagen structure was imaged using SHG microscopy. The degree of CFC was determined by measuring the percentage difference between the length of the collagen fiber and the linear distance traveled. CFC was measured in the central anterior and posterior CXL region, the peripheral non-crosslinked region in the same cornea, and the central cornea of the non-crosslinked contralateral eye. No change in corneal thickness was detected after one month, however the stromal thickness surpassed its original baseline thickness at three months by 25.9 μm. Corneal haze peaked at one month and then began to clear. Increased CAF was detected in all CXL corneas, localized to the anterior stroma and extending to 42.4 ± 3.4% and 47.7 ± 7.6% of the corneal thickness at one and three months. There was a significant (P < 0.05) reduction in CFC in the CAF region in all eyes averaging 1.007 ± 0.006 and 1.009 ± 0.005 in one and three month samples compared to 1.017 ± 0.04 and 1.016 ± 0.06 for controls. These results indicate that there is a significant reduction in collagen crimping following UVA CXL of approximately 1%. One possible explanation for this loss of crimping could be shortening of the collagen fibers over the CXL region.
登录
查看更多内容
影响因子：
3.4
作者：
Quantock, Andrew J.;Winkler, Moritz;Parfitt, Geraint J.;Young, Robert D.;Brown, Donald J.;Boote, Craig;Jester, James V.
通讯作者：
Jester, James V.
影响因子：
3.8
作者：
Pierlot, Caitlin M.;Lee, J. Michael;Wells, Sarah M.
通讯作者：
Wells, Sarah M.
影响因子：
1.4
作者：
DOUGHTY, MJ
通讯作者：
DOUGHTY, MJ
影响因子：
4.4
作者：
Sondergaard, Anders P.;Ivarsen, Anders;Hjortdal, Jesper
通讯作者：
Hjortdal, Jesper
影响因子：
2.2
作者：
Malik S;Humayun S;Nayyar S;Ishaq M
通讯作者：
Ishaq M