Direct analysis of Holliday junction resolving enzyme in a DNA origami nanostructure.
Direct analysis of Holliday junction resolving enzyme in a DNA origami nanostructure.
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DOI：
10.1093/nar/gku320
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发表时间：
2014-06
影响因子：
14.9
通讯作者：
Takeyasu K
中科院分区：
文献类型：
--
作者：
Suzuki Y;Endo M;Cañas C;Ayora S;Alonso JC;Sugiyama H;Takeyasu K
霍利迪连接（HJ）的拆分是完成同源重组的基础步骤。HJ分解酶（分解酶）在结合和先前切割时扭曲连接结构，提高了酶的反应性可能受到连接处的特定几何形状和拓扑结构影响的可能性。在这里，我们采用了一种DNA折纸纳米支架，其中HJ的每个臂通过碱基对杂交被拴在一起，使我们能够通过调整DNA臂的长度来使连接核心变得灵活或不灵活。柔性和非柔性连接都与枯草芽孢杆菌RecU HJ解离酶结合，而只有柔性连接被该酶有效地分解成两个双链体。这一结果表明，对于反应的进行，结核的结构延展性的重要性。此外，通过分析反应产物的形态，解决了RecU介导的反应的切割偏好。
Holliday junction (HJ) resolution is a fundamental step for completion of homologous recombination. HJ resolving enzymes (resolvases) distort the junction structure upon binding and prior cleavage, raising the possibility that the reactivity of the enzyme can be affected by a particular geometry and topology at the junction. Here, we employed a DNA origami nano-scaffold in which each arm of a HJ was tethered through the base-pair hybridization, allowing us to make the junction core either flexible or inflexible by adjusting the length of the DNA arms. Both flexible and inflexible junctions bound to Bacillus subtilis RecU HJ resolvase, while only the flexible junction was efficiently resolved into two duplexes by this enzyme. This result indicates the importance of the structural malleability of the junction core for the reaction to proceed. Moreover, cleavage preferences of RecU-mediated reaction were addressed by analyzing morphology of the reaction products.
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影响因子：
14.9
作者：
Górecka KM;Komorowska W;Nowotny M
通讯作者：
Nowotny M
DOI：
10.1073/pnas.92.12.5635
发表时间：
1995-06-06
影响因子：
11.1
作者：
BENNETT, RJ;WEST, SC
通讯作者：
WEST, SC
影响因子：
5.4
作者：
Yokokawa, Masatoshi;Takeyasu, Kunio
通讯作者：
Takeyasu, Kunio
影响因子：
14.9
作者：
Rajendran A;Endo M;Hidaka K;Tran PL;Mergny JL;Sugiyama H
通讯作者：
Sugiyama H
影响因子：
4.8
作者：
Postow, L;Ullsperger, C;Cozzarelli, NR
通讯作者：
Cozzarelli, NR