The complete structure of the cucumber (Cucumis sativus L.) chloroplast genome: its composition and comparative analysis.

The complete structure of the cucumber (Cucumis sativus L.) chloroplast genome: its composition and comparative analysis.
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DOI:
10.2478/s11658-007-0029-7
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发表时间:
2007
影响因子:
8.3
通讯作者:
Malepszy S
Malepszy S
中科院分区:
生物学1区
文献类型:
--
作者:
Plader W;Yukawa Y;Sugiura M;Malepszy S

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黄瓜(C. sativus L.变种Borszczagowski)叶绿体基因组的测定。该基因组由155,293 bp组成,其中包含一对25,191 bp的反向重复序列,这对反向重复序列被两个单拷贝区域分开,一个小的18,222 bp区域和一个大的86,688 bp区域。黄瓜叶绿体基因组包含130个已知基因,其中蛋白质编码基因89个,核糖体RNA基因8个(4种rRNA),tRNA基因37个(30种tRNA),其中18个位于反向重复区。在这些基因中,16个含有一个内含子,2个基因和1个ycf含有2个内含子。已经鉴定了21个形成茎环结构的小倒位,范围从18到49 bp。其中8个与其他物种相似,而8个似乎是黄瓜特有的。对ycf 2和ycf 15以及与其他叶绿体基因组的整体结构进行了详细的比较。
The complete nucleotide sequence of the cucumber (C. sativus L. var. Borszczagowski) chloroplast genome has been determined. The genome is composed of 155,293 bp containing a pair of inverted repeats of 25,191 bp, which are separated by two single-copy regions, a small 18,222-bp one and a large 86,688-bp one. The chloroplast genome of cucumber contains 130 known genes, including 89 protein-coding genes, 8 ribosomal RNA genes (4 rRNA species), and 37 tRNA genes (30 tRNA species), with 18 of them located in the inverted repeat region. Of these genes, 16 contain one intron, and two genes and one ycf contain 2 introns. Twenty-one small inversions that form stem-loop structures, ranging from 18 to 49 bp, have been identified. Eight of them show similarity to those of other species, while eight seem to be cucumber specific. Detailed comparisons of ycf2 and ycf15, and the overall structure to other chloroplast genomes were performed.
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