A role for CCR4 in development of mature circulating cutaneous T helper memory cell populations.
A role for CCR4 in development of mature circulating cutaneous T helper memory cell populations.
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DOI：
10.1084/jem.20041059
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发表时间：
2005-04-04
影响因子：
15.3
通讯作者：
Campbell, JJ
中科院分区：
文献类型：
--
作者：
Baekkevold, ES;Wurbel, MA;Kivisäkk, P;Wain, CM;Power, CA;Haraldsen, G;Campbell, JJ
趋化因子受体CCR 4的表达与特化的皮肤记忆性T辅助（Th）淋巴细胞向皮肤的运输密切相关。然而，目前尚不清楚CCR 4本身是否参与皮肤Th细胞群的发育。我们已经通过竞争性骨髓（BM）重建试验解决了这个问题;允许相同数量的来自CCR 4 +/+和CCR 4 −/−供体的BM细胞在RAG-1−/−宿主中并排发育。来自两种供体类型的细胞同样良好地发育成B细胞、初始CD 8 T细胞、初始CD 4 T细胞、干扰素-γ+ Th 1细胞和白细胞介素-4 + Th 2细胞。与此形成鲜明对比的是，循环皮肤记忆Th细胞（即，E-selectin ligand+ [E-lig+]）来源于CCR 4 +/+供体的可能性是来源于CCR 4 −/−供体的四倍多。大多数这种效应存在于E-lig+ Th群体的CD 103+亚群中，其中供体CCR 4 +/+细胞的数量可以超过CCR 4 −/−细胞>12倍。对于α4β7+肠道记忆Th细胞或CD 103 +/E-lig− Th细胞，未观察到类似的效应。我们的结论是，CCR 4的表达提供了一个竞争优势，皮肤Th细胞，无论是通过参与他们的发展从幼稚Th细胞，或通过优先保持他们的记忆人口随着时间的推移。
Expression of the chemokine receptor CCR4 is strongly associated with trafficking of specialized cutaneous memory T helper (Th) lymphocytes to the skin. However, it is unknown whether CCR4 itself participates in the development of cutaneous Th populations. We have addressed this issue via competitive bone marrow (BM) reconstitution assays; equal numbers of BM cells from CCR4+/+ and CCR4−/− donors were allowed to develop side-by-side within RAG-1−/− hosts. Cells from both donor types developed equally well into B cells, naive CD8 T cells, naive CD4 T cells, interferon-γ+ Th1 cells, and interleukin-4+ Th2 cells. In marked contrast, circulating cutaneous memory Th cells (i.e., E-selectin ligand+ [E-lig+]) were more than fourfold more likely to be derived from CCR4+/+ donors than from CCR4−/− donors. Most of this effect resides within the CD103+ subset of the E-lig+ Th population, in which donor CCR4+/+ cells can outnumber CCR4−/− cells by >12-fold. No similar effect was observed for α4β7+ intestinal memory Th cells or CD103+/E-lig− Th cells. We conclude that CCR4 expression provides a competitive advantage to cutaneous Th cells, either by participating in their development from naive Th cells, or by preferentially maintaining them within the memory population over time.
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DOI：
10.1073/pnas.192162899
发表时间：
2002-10-01
影响因子：
11.1
作者：
Lehmann, J;Huehn, J;Hamann, A
通讯作者：
Hamann, A
影响因子：
15.9
作者：
Kim, CH;Rott, L;Butcher, EC
通讯作者：
Butcher, EC
DOI：
10.1084/jem.194.10.1541
发表时间：
2001-11-19
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Reiss Y;Proudfoot AE;Power CA;Campbell JJ;Butcher EC
通讯作者：
Butcher EC
影响因子：
64.8
作者：
Mora, JR;Bono, MR;von Andrian, UH
通讯作者：
von Andrian, UH
影响因子：
5.4
作者：
Mackay, CR;Andrew, DP;Butcher, EC
通讯作者：
Butcher, EC