Versatile Bottom-Up Synthesis of Tethered Bilayer Lipid Membranes on Nanoelectronic Biosensor Devices.
Versatile Bottom-Up Synthesis of Tethered Bilayer Lipid Membranes on Nanoelectronic Biosensor Devices.
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DOI：
10.1021/acsami.7b00268
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发表时间：
2017-05-03
影响因子：
9.5
通讯作者：
Burke PJ
中科院分区：
文献类型：
--
作者：
Zhou W;Burke PJ
将纳米电子器件与细胞膜连接可以实现对基本生物过程（如信号转导、电生理学和输入/输出控制）的多路检测，甚至可以达到单离子通道水平，这可以导致药理学和临床诊断中的各种应用。因此，有必要了解和控制器件与脂质双层膜之间的化学和电学界面。在这里，我们开发了一个简单的自下而上的方法来组装拴系双层类脂膜（tBLMs）的硅晶片和载玻片上，使用共价拴系连接化学硅烷功能化的基础上，然后逐步堆叠的两个其他功能分子的积木（寡聚（乙二醇）（PEG）和脂质）。使用标准囊泡融合过程来完成双层形成。单层合成方案包括三个公认的化学反应：自组装，环氧-胺反应，和EDC/NHS交联反应。所有这三种反应都是容易和简单的，并且可以在许多研究实验室中容易地实施，基于常见的市售前体，使用温和的反应条件。寡聚PEG作为亲水性间隔物，在形成均质双层膜中起关键作用。为了探索这种方法的广泛适用性，我们进一步证明了在三种常见的（纳米）电子生物传感器设备上形成tBLMs：铟锡氧化物涂层玻璃，硅纳米器件和玻璃上的高密度单壁碳纳米管（SWNT）网络。更重要的是，我们将阿来霉素引入tBLMs中，利用tBLMs/SWNT网络晶体管混合平台实现了高灵敏度、高时间分辨率的单离子通道活性实时记录。这种方法可以在纳米电子器件的氧化物层上提供共价键合的脂质涂层，这将使纳米电子接口到电生理学的新兴领域中的各种应用成为可能。
Interfacing nanoelectronic devices with cell membranes can enable multiplexed detection of fundamental biological processes (such as signal transduction, electrophysiology, and import/export control) even down to the single ion channel level, which can lead to a variety of applications in pharmacology and clinical diagnosis. Therefore, it is necessary to understand and control the chemical and electrical interface between the device and the lipid bilayer membrane. Here, we develop a simple bottom-up approach to assemble tethered bilayer lipid membranes (tBLMs) on silicon wafers and glass slides, using a covalent tether attachment chemistry based on silane functionalization, followed by step-by-step stacking of two other functional molecular building blocks (oligo-poly(ethylene glycol) (PEG) and lipid). A standard vesicle fusion process was used to complete the bilayer formation. The monolayer synthetic scheme includes three well-established chemical reactions: self-assembly, epoxy-amine reaction, and EDC/NHS cross-linking reaction. All three reactions are facile and simple and can be easily implemented in many research labs, on the basis of common, commercially available precursors using mild reaction conditions. The oligo-PEG acts as the hydrophilic spacer, a key role in the formation of a homogeneous bilayer membrane. To explore the broad applicability of this approach, we have further demonstrated the formation of tBLMs on three common classes of (nano)electronic biosensor devices: indium-tin oxide-coated glass, silicon nanoribbon devices, and high-density single-walled carbon nanotubes (SWNT) networks on glass. More importantly, we incorporated alemethicin into tBLMs and realized the real-time recording of single ion channel activity with high sensitivity and high temporal resolution using the tBLMs/SWNT network transistor hybrid platform. This approach can provide a covalently bonded lipid coating on the oxide layer of nanoelectronic devices, which will enable a variety of applications in the emerging field of nanoelectronic interfaces to electrophysiology.
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DOI：
10.1021/la401132c
发表时间：
2013-07-09
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Budvytyte R;Valincius G;Niaura G;Voiciuk V;Mickevicius M;Chapman H;Goh HZ;Shekhar P;Heinrich F;Shenoy S;Lösche M;Vanderah DJ
通讯作者：
Vanderah DJ
影响因子：
38.3
作者：
通讯作者：
--
影响因子：
15.6
作者：
Karakoti, Ajay Singh;Shukla, Ritesh;Singh, Sanjay
通讯作者：
Singh, Sanjay
影响因子：
9.9
作者：
Ariza, MJ;Rodríguez-Castellón, E;Oleinikova, M
通讯作者：
Oleinikova, M
影响因子：
4.5
作者：
Demirel, G.;Caglayan, M. O.;Piskin, E.
通讯作者：
Piskin, E.