Impaired Rhodopsin Generation in the Rat Model of Diabetic Retinopathy.
Impaired Rhodopsin Generation in the Rat Model of Diabetic Retinopathy.
复制标题
DOI：
10.1016/j.ajpath.2017.06.007
复制
发表时间：
2017-10
期刊：
影响因子：
--
通讯作者：
Ma JX
中科院分区：
文献类型：
--
作者：
Malechka VV;Moiseyev G;Takahashi Y;Shin Y;Ma JX
糖尿病视网膜病变是糖尿病的常见并发症。在微血管病变变得明显之前，糖尿病患者会经历暗适应、对比敏感度和颜色感知的功能缺陷。在此，我们评估了糖尿病患者眼部神经视网膜功能和类维生素A代谢的早期变化。与非糖尿病对照组相比，链脲佐菌素诱导的糖尿病大鼠在糖尿病诱导后 4 个月表现出暗视和明视视网膜电图反应的 a 和 b 波振幅降低。尽管蛋白质印迹分析显示视蛋白表达没有差异，但糖尿病视网膜中视紫红质含量降低，如吸光度差异所示。一致的是，与对照组相比，糖尿病患者视网膜中视色素发色团 11-cis-retinal 的水平显着降低，表明存在类维生素A缺乏。在视觉周期蛋白中，糖尿病大鼠中的光感受器间视黄酸结合蛋白和视黄酸 6 蛋白刺激的水平显着低于非糖尿病对照组。同样，糖尿病大鼠中视黄醇结合蛋白 4 和类视黄醇的血清水平显着降低。总体而言，这些结果表明 1 型糖尿病患者眼睛中的类视黄醇代谢受损，从而导致早期糖尿病患者视色素生成不足和神经视网膜功能障碍。
Diabetic retinopathy is a common complication of diabetes mellitus. Diabetic patients experience functional deficits in dark adaptation, contrast sensitivity, and color perception before microvascular pathologies become apparent. Herein, we evaluated early changes in neural retinal function and in retinoid metabolism in the eye in diabetes. Streptozotocin-induced diabetic rats showed decreased a- and b-wave amplitudes of scotopic and photopic electroretinography responses 4 months after diabetes induction compared to nondiabetic controls. Although Western blot analysis revealed no difference in opsin expression, rhodopsin content was decreased in diabetic retinas, as shown by a difference in absorbance. Consistently, levels of 11-cis-retinal, the chromophore for visual pigments, were significantly lower in diabetic retinas compared to those in controls, suggesting a retinoid deficiency. Among visual cycle proteins, interphotoreceptor retinoid-binding protein and stimulated by retinoic acid 6 protein showed significantly lower levels in diabetic rats than those in nondiabetic controls. Similarly, serum levels of retinol-binding protein 4 and retinoids were significantly lower in diabetic rats. Overall, these results suggest that retinoid metabolism in the eye is impaired in type 1 diabetes, which leads to deficient generation of visual pigments and neural retinal dysfunction in early diabetes.
登录
查看更多内容
影响因子：
4.4
作者：
Betts-Obregon, Brandi S.;Gonzalez-Fernandez, Federico;Tsin, Andrew T.
通讯作者：
Tsin, Andrew T.
影响因子：
3.3
作者：
Gonzalez-Fernandez, Federico;Betts-Obregon, Brandi;Tsin, Andrew T.
通讯作者：
Tsin, Andrew T.
影响因子：
6
作者：
Granado, F;Olmedilla, B;Rojas-Hidalgo, E
通讯作者：
Rojas-Hidalgo, E
视网膜类似（视觉）周期的化学。
影响因子：
62.1
作者：
Kiser, Philip D.;Golczak, Marcin;Palczewski, Krzysztof
通讯作者：
Palczewski, Krzysztof
影响因子：
4.4
作者：
Fan, Jie;Rohrer, Baerbel;Crouch, Rosalie K.
通讯作者：
Crouch, Rosalie K.