Real-time adsorption and action of expansin on cellulose.
Real-time adsorption and action of expansin on cellulose.
复制标题
扩张蛋白对纤维素的实时吸附和作用
DOI：
10.1186/s13068-018-1318-2
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发表时间：
2018
影响因子：
6.3
通讯作者：
He Z
中科院分区：
文献类型：
--
作者：
Duan Y;Ma Y;Zhao X;Huang R;Su R;Qi W;He Z
生物预处理是一种环境友好的方法，用于破坏木质纤维素的顽固结构，从而提高其水解效率。膨胀素及膨胀素类蛋白在水解过程中与纤维素酶协同作用。对膨胀素家族蛋白的吸附行为及作用机制进行系统分析，可为利用膨胀素开发纤维素底物的高效预处理方法提供依据。
利用耗散型石英晶体微天平实时监测了不同条件下枯草芽孢杆菌膨胀素（BsEXLX1）在纤维素膜上的吸附情况。建立了一个模型来描述BsEXLX1在膜上的吸附。高温提高了初始吸附速率，但降低了吸附在纤维素上的BsEXLX1的最大量。低浓度的非离子表面活性剂（聚乙二醇4000和吐温80）增强了BsEXLX1的吸附；而高浓度则有相反的效果。然而，十二烷基硫酸钠在低浓度和高浓度时均抑制吸附。我们还研究了BsEXLX1吸附时纤维素的结构变化，发现BsEXLX1吸附降低了结晶度指数，破坏了氢键，并增加了纤维素的表面积，表明底物更易被蛋白作用。
这些结果增进了我们对膨胀素与纤维素之间相互作用的理解，并为将膨胀素处理作为一种有前景的策略来提高木质纤维素的酶水解提供了证据。
本文的网络版（10.1186/s13068 - 2018 - 1318 - 2）包含补充材料，可供授权用户使用。
Biological pretreatment is an environmentally safe method for disrupting recalcitrant structures of lignocellulose and thereby improving their hydrolysis efficiency. Expansin and expansin-like proteins act synergistically with cellulases during hydrolysis. A systematic analysis of the adsorption behavior and mechanism of action of expansin family proteins can provide a basis for the development of highly efficient pretreatment methods for cellulosic substrates using expansins. Adsorption of Bacillus subtilis expansin (BsEXLX1) onto cellulose film under different conditions was monitored in real time using a quartz crystal microbalance with dissipation. A model was established to describe the adsorption of BsEXLX1 onto the film. High temperatures increased the initial adsorption rate while reducing the maximum amount of BsEXLX1 adsorbed onto the cellulose. Non-ionic surfactants (polyethylene glycol 4000 and Tween 80) at low concentrations enhanced BsEXLX1 adsorption; whereas, high concentrations had the opposite effect. However, sodium dodecyl sulfate inhibited adsorption at both low and high concentrations. We also investigated the structural changes of cellulose upon BsEXLX1 adsorption and found that BsEXLX1 adsorption decreased the crystallinity index, disrupted hydrogen bonding, and increased the surface area of cellulose, indicating greater accessibility of the substrate to the protein. These results increase our understanding of the interaction between expansin and cellulose, and provide evidence for expansin treatment as a promising strategy to enhance enzymatic hydrolysis of lignocellulose. The online version of this article (10.1186/s13068-018-1318-2) contains supplementary material, which is available to authorized users.
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来自微生物世界的细菌膨胀蛋白和相关蛋白质。
影响因子：
5
作者：
Georgelis, Nikolaos;Nikolaidis, Nikolas;Cosgrove, Daniel J.
通讯作者：
Cosgrove, Daniel J.
影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Radke, Clayton J.
影响因子：
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作者：
Borjesson, Johan;Peterson, Ragna;Tjerneld, Folke
通讯作者：
Tjerneld, Folke