Mitochondrial dysfunction is involved in the toxic activity of boric acid against Saprolegnia.
Mitochondrial dysfunction is involved in the toxic activity of boric acid against Saprolegnia.
复制标题
线粒体功能障碍参与硼酸对腐生菌的毒性活性。
DOI：
10.1371/journal.pone.0110343
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Skaar I
中科院分区：
文献类型：
--
作者：
Ali SE;Thoen E;Evensen Ø;Wiik-Nielsen J;Gamil AA;Skaar I
在过去的几十年里，特别是在禁止使用孔雀石绿之后，腐殖质感染的发病率显著增加。这些感染通常与鲑鱼养殖场和孵化场的高经济损失有关。最近在体外和体内条件下研究了硼酸控制该疾病的使用，但其对鱼致病性腐毛虫的可能作用方式尚不清楚。在这项研究中，我们探索了在处理后4-24小时暴露于硼酸（1 g/L）后腐烂菌孢子/菌丝的转化。利用透射电子显微镜（TEM）检测了孢子的早期变化。随机选择的约20%的孢子在处理4小时后观察到线粒体变性是最明显的迹象。我们还利用共聚焦激光扫描显微镜（CLSM）研究了处理对核分裂、线粒体活性和功能的影响。荧光显微镜还检测了处理对线粒体膜电位和活性氧形成的影响。此外，利用MTS法检测了与线粒体酶活性相关的处理孢子的生存能力和增殖能力。所有获得的数据都指向了治疗后线粒体结构、膜电位和酶活性的变化。我们发现硼酸在测试浓度下对孢子膜的完整性没有影响。因此，线粒体功能障碍可能与硼酸对孢子虫的毒性作用有关。
There has been a significant increase in the incidence of Saprolegnia infections over the past decades, especially after the banning of malachite green. Very often these infections are associated with high economic losses in salmonid farms and hatcheries. The use of boric acid to control the disease has been investigated recently both under in vitro and in vivo conditions, however its possible mode of action against fish pathogenic Saprolegnia is not known. In this study, we have explored the transformation in Saprolegnia spores/hyphae after exposure to boric acid (1 g/L) over a period 4–24 h post treatment. Using transmission electron microscopy (TEM), early changes in Saprolegnia spores were detected. Mitochondrial degeneration was the most obvious sign observed following 4 h treatment in about 20% of randomly selected spores. We also investigated the effect of the treatment on nuclear division, mitochondrial activity and function using confocal laser scanning microscopy (CLSM). Fluorescence microscopy was also used to test the effect of treatment on mitochondrial membrane potential and formation of reactive oxygen species. Additionally, the viability and proliferation of treated spores that correlated to mitochondrial enzymatic activity were tested using an MTS assay. All obtained data pointed towards changes in the mitochondrial structure, membrane potential and enzymatic activity following treatment. We have found that boric acid has no effect on the integrity of membranes of Saprolegnia spores at concentrations tested. It is therefore likely that mitochondrial dysfunction is involved in the toxic activity of boric acid against Saprolegnia spp.
登录
查看更多内容
影响因子：
3.7
作者：
Ali SE;Thoen E;Evensen Ø;Skaar I
通讯作者：
Skaar I
影响因子：
3.7
作者：
Lopes G;Pinto E;Andrade PB;Valentão P
通讯作者：
Valentão P
影响因子：
16.2
作者：
CORY, AH;OWEN, TC;CORY, JG
通讯作者：
CORY, JG
影响因子：
3
作者：
Ruy, Fernando;Vercesi, Anibal E.;Kowaltowski, Alicia J.
通讯作者：
Kowaltowski, Alicia J.
DOI：
10.1139/b80-021
发表时间：
1980-01-01
期刊：
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE
影响因子：
--
作者：
BEAKES, GW
通讯作者：
BEAKES, GW