Boric acid inhibits germination and colonization of Saprolegnia spores in vitro and in vivo.
Boric acid inhibits germination and colonization of Saprolegnia spores in vitro and in vivo.
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DOI：
10.1371/journal.pone.0091878
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Skaar I
中科院分区：
文献类型：
--
作者：
Ali SE;Thoen E;Evensen Ø;Skaar I
水霉属感染在淡水鱼及其卵中造成严重的经济损失。禁止使用孔雀石绿色增加了寻找有效的替代治疗方法来控制疾病的需求。在本研究中，我们调查了硼酸控制鲑鱼卵和卵黄囊鱼苗中的水霉病的能力。在离体培养条件下，0.2g/L以上浓度的硼酸对水霉孢子活力和菌丝生长均有抑制作用，而0.8g/L的硼酸对水霉孢子萌发和菌丝生长完全抑制。在使用大西洋鲑鱼眼卵的体内实验中，水霉病在连续暴露期间由浓度范围为0.2-1.4 g/L的硼酸控制，在间歇暴露期间由浓度范围为1.0-4.0 g/L的硼酸控制。在水霉病自然爆发期间，对连续暴露于0.5g/L硼酸的鲑鱼卵黄囊鱼苗观察到相同的效果。在实验期间，在蛋或鱼苗中未观察到对孵化率和存活力的负面影响，这表明在所有测试浓度下使用都是安全的。体内试验后记录的高孵化率和存活率表明，硼酸是预防和控制水霉病的候选物。
Saprolegnia infections cause severe economic losses among freshwater fish and their eggs. The banning of malachite green increased the demand for finding effective alternative treatments to control the disease. In the present study, we investigated the ability of boric acid to control saprolegniosis in salmon eggs and yolk sac fry. Under in vitro conditions, boric acid was able to decrease Saprolegnia spore activity and mycelial growth in all tested concentrations above 0.2 g/L, while complete inhibition of germination and growth was observed at a concentration of 0.8 g/L. In in vivo experiments using Atlantic salmon eyed eggs, saprolegniosis was controlled by boric acid at concentrations ranging from 0.2–1.4 g/L during continuous exposure, and at 1.0–4.0 g/L during intermittent exposure. The same effect was observed on salmon yolk sac fry exposed continuously to 0.5 g/L boric acid during the natural outbreak of saprolegniosis. During the experiments no negative impact with regard to hatchability and viability was observed in either eggs or fry, which indicate safety of use at all tested concentrations. The high hatchability and survival rates recorded following the in vivo testing suggest that boric acid is a candidate for prophylaxis and control of saprolegniosis.
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影响因子：
2.5
作者：
Thoen, E.;Evensen, O.;Skaar, I.
通讯作者：
Skaar, I.
影响因子：
3.3
作者：
Ali, Shimaa E.;Thoen, Even;Skaar, Ida
通讯作者：
Skaar, Ida
DOI：
10.1079/9781845935542.0669
发表时间：
2011-01-01
期刊：
FISH DISEASES AND DISORDERS, VOL 3: VIRAL, BACTERIAL AND FUNGAL INFECTIONS, 2ND EDITION
影响因子：
--
作者：
Bruno, David W.;Van West, Pieter;Beakes, Gordon W.
通讯作者：
Beakes, Gordon W.
影响因子：
5.2
作者：
De Seta, Francesco;Schmidt, Martin;Larsen, Bryan
通讯作者：
Larsen, Bryan
影响因子：
--
作者：
Magaraggia, Michela;Faccenda, Filippo;Jori, Giulio
通讯作者：
Jori, Giulio