Ancient dental calculus preserves signatures of biofilm succession and interindividual variation independent of dental pathology.
Ancient dental calculus preserves signatures of biofilm succession and interindividual variation independent of dental pathology.
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DOI：
10.1093/pnasnexus/pgac148
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发表时间：
2022-09
期刊：
影响因子：
--
通讯作者：
Warinner, Christina
中科院分区：
文献类型：
--
作者：
Velsko, Irina M.;Semerau, Lena;Inskip, Sarah A.;Garcia-Collado, Maite I.;Ziesemer, Kirsten;Ruber, Maria Serrano;de Lugo Enrich, Luis Benitez;Molero Garcia, Jesus Manuel;Gallego Valle, David;Pena Ruiz, Ana Cristina;Salazar-Garcia, Domingo C.;Hoogland, Menno L. P.;Warinner, Christina
牙石保存口腔微生物，使口腔微生物组和健康的比较研究随着时间的推移。然而，小样本量和有限的牙齿健康元数据阻碍了以健康为重点的调查。在这里，我们调查了吸烟和牙结石微生物之间的关系。通过宏基因组学分析了来自19世纪世纪Middenbeemster骨骼收集（荷兰）的75个个体的牙结石。系统记录人口统计学和牙齿健康参数，包括管道缺口的存在/数量。还分析了引进烟草前后欧洲人口的比较数据集。将结石物种特征与口腔病理学进行比较，以检查微生物群落，吸烟行为和口腔健康状况之间的关联。Middenbeemster个体的口腔健康状况相对较差，牙周病、龋齿、重度牙石沉积和死前牙齿脱落的患病率较高。烟斗切口和牙齿病理或微生物物种组成之间没有关联，被发现。引入烟草之前和之后的结石样本显示出高度相似的物种概况。观察到的个体间微生物组差异与先前描述的从旧石器时代晚期到现在的人类种群变化一致。牙石可能不像牙菌斑那样明显地保存健康和疾病状态的微生物指标。
Dental calculus preserves oral microbes, enabling comparative studies of the oral microbiome and health through time. However, small sample sizes and limited dental health metadata have hindered health-focused investigations to date. Here, we investigate the relationship between tobacco pipe smoking and dental calculus microbiomes. Dental calculus from 75 individuals from the 19th century Middenbeemster skeletal collection (Netherlands) were analyzed by metagenomics. Demographic and dental health parameters were systematically recorded, including the presence/number of pipe notches. Comparative data sets from European populations before and after the introduction of tobacco were also analyzed. Calculus species profiles were compared with oral pathology to examine associations between microbiome community, smoking behavior, and oral health status. The Middenbeemster individuals exhibited relatively poor oral health, with a high prevalence of periodontal disease, caries, heavy calculus deposits, and antemortem tooth loss. No associations between pipe notches and dental pathologies, or microbial species composition, were found. Calculus samples before and after the introduction of tobacco showed highly similar species profiles. Observed interindividual microbiome differences were consistent with previously described variation in human populations from the Upper Paleolithic to the present. Dental calculus may not preserve microbial indicators of health and disease status as distinctly as dental plaque.
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影响因子：
11
作者：
Duran-Pinedo, Ana E.;Chen, Tsute;Frias-Lopez, Jorge
通讯作者：
Frias-Lopez, Jorge
影响因子：
7.7
作者：
Beghini F;McIver LJ;Blanco-Míguez A;Dubois L;Asnicar F;Maharjan S;Mailyan A;Manghi P;Scholz M;Thomas AM;Valles-Colomer M;Weingart G;Zhang Y;Zolfo M;Huttenhower C;Franzosa EA;Segata N
通讯作者：
Segata N
影响因子：
5.6
作者：
Beghini, Francesco;Renson, Audrey;Waldron, Levi
通讯作者：
Waldron, Levi
影响因子：
15.5
作者：
Davis, Nicole M;Proctor, Diana M;Callahan, Benjamin J
通讯作者：
Callahan, Benjamin J
影响因子：
4.3
作者：
Briscoe L;Balliu B;Sankararaman S;Halperin E;Garud NR
通讯作者：
Garud NR