Dynamic transcriptome and phytohormone profiling along the time of light exposure in the mesocotyl of rice seedling.
Dynamic transcriptome and phytohormone profiling along the time of light exposure in the mesocotyl of rice seedling.
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水稻幼苗中胚轴随光照时间的动态转录组和植物激素分析
DOI：
10.1038/s41598-017-12326-2
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发表时间：
2017-09-20
影响因子：
4.6
通讯作者：
Luo L
中科院分区：
文献类型：
--
作者：
Feng F;Mei H;Fan P;Li Y;Xu X;Wei H;Yan M;Luo L
中胚轴伸长是影响水稻直播栽培出苗和成苗的重要性状，光照后中胚轴伸长立即受到抑制。对中胚轴生长受光抑制的分子基础和生物学过程的详细研究可能会为控制这一性状的关键因素提供有用的信息。在这里，我们监测了转录组和内源性植物激素的变化，特别是在延长中胚轴响应光暴露的时间过程。结果显示，974个转录本的表达差异显著（FDR < 0.05，|log2（L/D）|≥2）光照后。差异表达基因大多与激素反应有关。利用KEGG系统对代谢途径的分析表明，植物激素信号转导、α-亚麻酸代谢和二萜类化合物合成是光抑制中胚轴生长的关键过程。与DEG结果一致，内源IAA、tZ和GA 3含量显著降低，JA水平显著升高，表明光照通过降低IAA、tZ和GA 3含量和/或升高JA水平抑制水稻中胚轴生长。本研究结果丰富了水稻中胚轴伸长调控基因和激素的研究内容，为进一步开展相关基因的研究和培育耐深播水稻新品种奠定了基础。
Mesocotyl elongation is an important trait influencing seedling emergence and establishment in rice direct-seeding cultivation and is immediately inhibited after light exposure. Detailed researches on the molecular basis and biological processes underlying light repression of mesocotyl growth could probably provide useful information for key factors controlling this trait. Here we monitored the transcriptome and endogenous phytohormone changes specifically in the elongating mesocotyl in response to light exposure with a time-course. It was revealed that 974 transcripts were significantly differentially expressed (FDR < 0.05, |log2(L/D) | ≥2) after light exposure. Most of the differential expression genes associated with the responses to hormone. Metabolic pathway analysis using the KEGG system suggested plant hormone signal transduction, α-linolenic acid metabolism and diterpenoid biosynthesis were critical processes of mesocotyl growth inhibited by light. Consistent with DEGs, the endogenous IAA, tZ and GA3content was significantly reduced while JA level was dramatically increased, which indicated that light inhibited rice mesocotyl growth through decreasing IAA, tZ and GA3content and/or increasing JA level. The present results enriched our knowledge about the genes and phytohormones regulating mesocotyl elongation in rice, which may help improve future studies on associated genes and develop new varieties tolerance to deep sowing.
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影响因子：
3.7
作者：
Shi D;Zheng X;Li L;Lin W;Xie W;Yang J;Chen S;Jin W
通讯作者：
Jin W
影响因子：
7.4
作者：
Cona, A;Cenci, F;Angelini, R
通讯作者：
Angelini, R
使用单 SPE 纯化和同位素稀释，通过 UPLC-MS/MS 简单、快速、同时测定受伤番茄中的多种含羧基植物激素
影响因子：
1.6
作者：
Fu, Jihong;Chu, Jinfang;Yan, Cunyu
通讯作者：
Yan, Cunyu
影响因子：
7.2
作者：
Kim, JH;Choi, DS;Kende, H
通讯作者：
Kende, H
影响因子：
1.6
作者：
Nakamura, Yuko;Kato, Takahiko;Mizuno, Takeshi
通讯作者：
Mizuno, Takeshi