Role of long non-coding RNA-RNCR3 in atherosclerosis-related vascular dysfunction.
Role of long non-coding RNA-RNCR3 in atherosclerosis-related vascular dysfunction.
复制标题
长链非编码RNA-RNCR3在动脉粥样硬化相关血管功能障碍中的作用
DOI：
10.1038/cddis.2016.145
复制
发表时间：
2016-06-02
影响因子：
9
通讯作者：
Yan B
中科院分区：
文献类型：
--
作者：
Shan K;Jiang Q;Wang XQ;Wang YN;Yang H;Yao MD;Liu C;Li XM;Yao J;Liu B;Zhang YY;J Y;Yan B
动脉粥样硬化是最常见的血管疾病之一。内皮细胞（EC）功能障碍和血管平滑肌细胞（VSMC）增殖与动脉粥样硬化的发生有关。长链非编码rna （lncRNAs）与许多生物过程和人类疾病有关。我们发现lncRNA-RNCR3在ECs和vsmc中表达。体外ox-LDL处理后，RNCR3在小鼠和人主动脉粥样硬化病变以及体外培养的ECs和VSMCs中的表达均显著上调。RNCR3敲低可加速动脉粥样硬化的发展，加重高胆固醇血症和炎症因子释放，降低EC和VSMC在体内的增殖。RNCR3敲低还能抑制体外EC和VSMC的增殖和迁移，加速细胞凋亡的发展。RNCR3作为ceRNA，与Kruppel-like factor 2和miR-185-5p形成反馈回路，调控细胞功能。本研究表明，RNCR3在动脉粥样硬化中具有动脉粥样硬化保护作用，其干预是治疗动脉粥样硬化相关血管功能障碍的一种有希望的策略。
Atherosclerosis is one of the most common vascular disorders. Endothelial cell (EC) dysfunction and vascular smooth muscle cell (VSMC) proliferation contributes to the development of atherosclerosis. Long non-coding RNAs (lncRNAs) have been implicated in several biological processes and human diseases. Here we show that lncRNA-RNCR3 is expressed in ECs and VSMCs. RNCR3 expression is significantly upregulated in mouse and human aortic atherosclerotic lesions, and cultured ECs and VSMCs upon ox-LDL treatment in vitro. RNCR3 knockdown accelerates the development of atherosclerosis, aggravates hypercholesterolemia and inflammatory factor releases, and decreases EC and VSMC proliferation in vivo. RNCR3 knockdown also reduces the proliferation and migration, and accelerates apoptosis development of EC and VSMC in vitro. RNCR3 acts as a ceRNA, and forms a feedback loop with Kruppel-like factor 2 and miR-185-5p to regulate cell function. This study reveals that RNCR3 has an atheroprotective role in atherosclerosis, and its intervention is a promising strategy for treating atherosclerosis-related vascular dysfunction.
登录
查看更多内容
影响因子：
21.3
作者：
Hergenreider, Eduard;Heydt, Susanne;Dimmeler, Stefanie
通讯作者：
Dimmeler, Stefanie
影响因子：
20.1
作者：
Leung A;Trac C;Jin W;Lanting L;Akbany A;Sætrom P;Schones DE;Natarajan R
通讯作者：
Natarajan R
影响因子：
64.5
作者：
Huarte M;Guttman M;Feldser D;Garber M;Koziol MJ;Kenzelmann-Broz D;Khalil AM;Zuk O;Amit I;Rabani M;Attardi LD;Regev A;Lander ES;Jacks T;Rinn JL
通讯作者：
Rinn JL
starBase v2.0：从大规模 CLIP-Seq 数据中解码 miRNA-ceRNA、miRNA-ncRNA 和蛋白质-RNA 相互作用网络
影响因子：
14.9
作者：
Li JH;Liu S;Zhou H;Qu LH;Yang JH
通讯作者：
Yang JH
影响因子：
9.8
作者：
Nagy, Janice A.;Benjamin, Laura;Zeng, Huiyan;Dvorak, Ann M.;Dvorak, Harold F.
通讯作者：
Dvorak, Harold F.