Genome-wide analysis of circular RNAs in bovine cumulus cells treated with BMP15 and GDF9.
Genome-wide analysis of circular RNAs in bovine cumulus cells treated with BMP15 and GDF9.
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用 BMP15 和 GDF9 处理的牛卵丘细胞中环状 RNA 的全基因组分析
DOI：
10.1038/s41598-018-26157-2
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发表时间：
2018-05-21
影响因子：
4.6
通讯作者：
Zhang JB
中科院分区：
文献类型：
--
作者：
Fu Y;Jiang H;Liu JB;Sun XL;Zhang Z;Li S;Gao Y;Yuan B;Zhang JB
环状RNA（circRNA）是非编码RNA家族的重要成员，与动物生理相关的RNA近年来得到了广泛的研究。本研究旨在探讨circRNA在卵泡发育调控中的作用。我们构建了四个牛卵丘细胞 cDNA 文库，包括阴性对照组 (NC) 和 BMP15、GDF9 和 BMP15 + GDF9 处理组，并在 Illumina HiSeq Xten 平台上对文库进行了测序。我们鉴定了 1706 个 circRNA 并筛选了差异 circRNA 表达。我们对这些 circRNA 进行了生物信息学分析，并筛选了差异化 circRNA。宿主基因的功能注释和富集分析表明，差异化的circRNA与运动、繁殖、生物粘附、生长、节律过程、生物阶段和激素分泌有关。根据各组间circRNA表达差异，BMP15组有3个上调和6个下调的circRNA，GDF9组有12个上调和24个下调的circRNA。 BMP15 和 GDF9 的共同添加导致 15 个 circRNA 上调和 13 个 circRNA 下调。 circ_n/a_75、circ_12691_1和circ_n/a_303在BMP15和GDF9组以及BMP15 + GDF9组合组中均发生改变。我们关注这三种 circRNA，因为它们可能与 BMP15 和 GDF9 的累加效应相关。定量PCR分析表明，这3个circRNA的表达水平与测序结果一致。此外，circ_n/a_75和circ_n/a_303的靶miRNA、miR-339a、miR-2400和miR-30c在实验组中下调，这与circRNA的趋势相反。这些发现表明BMP15和GDF9可能通过circRNA作为miRNA海绵调控靶基因，从而调节牛卵丘细胞的状态并影响卵泡发育。
Circular RNAs (circRNAs) are important members of the non-coding RNA family, and those relating to animal physiologies have been widely studied in recent years. This study aimed to explore the roles of circRNAs in the regulation of follicular development. We constructed four bovine cumulus cell cDNA libraries, including a negative control group (NC) and groups treated with BMP15, GDF9 and BMP15 + GDF9, and we sequenced the libraries on the Illumina HiSeq Xten platform. We identified 1706 circRNAs and screened for differential circRNA expression. We conducted a bioinformatics analysis of these circRNAs and screened for differential circRNAs. Functional annotation and enrichment analysis of the host genes showed that the differential circRNAs were related to locomotion, reproduction, biological adhesion, growth, rhythmic processes, biological phases and hormone secretion. According to the differential expression of circRNA between groups, there were 3 up-regulated and 6 down-regulated circRNAs in the BMP15 group as well as 12 up-regulated and 24 down-regulated circRNAs in the GDF9 group. Co-addition of both BMP15 and GDF9 resulted in 15 up-regulated and 13 down-regulated circRNAs. circ_n/a_75,circ_12691_1 and circ_n/a_303 were altered in both the BMP15 and GDF9 groups as well as in the BMP15 + GDF9 combination group. We focused on these three circRNAs because they were potentially associated with the additive effect of BMP15 and GDF9. Quantitative PCR analysis showed that the expression levels of these three circRNAs were consistent with the sequencing results. In addition, the target miRNAs of circ_n/a_75 and circ_n/a_303, miR-339a, miR-2400 and miR-30c, were down-regulated in the experimental group, which was in contrast to the circRNAs trend. These findings demonstrated that BMP15 and GDF9 may regulate the target gene through circRNA, as a miRNA sponge, in order to regulate the status of bovine cumulus cells and affect follicular development.
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影响因子：
3.6
作者：
McIntosh, C. Joy;Lun, Stan;Juengel, Jennifer L.
通讯作者：
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通讯作者：
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