Exploring ALDH2 expression and immune infiltration in HNSC and its correlation of prognosis with gender or alcohol intake.
Exploring ALDH2 expression and immune infiltration in HNSC and its correlation of prognosis with gender or alcohol intake.
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探讨 HNSC 中 ALDH2 的表达和免疫浸润及其与性别或酒精摄入量的预后相关性
DOI：
10.1038/s41598-022-06244-1
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发表时间：
2022-02-15
影响因子：
4.6
通讯作者：
Cheng H
中科院分区：
文献类型：
--
作者：
Yao S;Yin X;Chen T;Chen W;Zuo H;Bi Z;Zhang X;Jing Y;Pang L;Cheng H
乙醛脱氢酶2点突变(ALDH2*2)是一种常见的人类基因变异，尤其是在东亚人中。然而，ALDH2在hNSC中的表达和作用机制尚不清楚。本研究旨在探讨ALDH2在hNSC中的临床意义及免疫特性。分析受试者工作特征曲线，评价ALDH2表达的诊断价值。用免疫组织化学方法检测ALDH2在正常组织和hNSC组织中的表达，并分析4个hNSC细胞系中ALDH2基因的表达。与正常组织相比，hNSC组织中ALDH2的表达显著降低(p <； )。高表达组预后优于低表达组(p <； 0.0 5)。GSEA表明，这些基因集与信号通路相关，包括JAK-STAT信号通路。出乎意料的是，我们发现了基于饮酒和男性性别的ALDH2对hNSC的显著预后影响。ALDH2的表达与免疫抑制剂的相关性表明，ALDH2具有改善hNSC肿瘤免疫功能的作用，ALDH2可能通过调节hNSC中T细胞的功能而发挥作用。此外，我们还开发了hNSC患者的预后诺模图，这表明在男性和酗酒的hNSC患者中，ALDH2的低表达表明预后较差。
The aldehyde dehydrogenase 2 point mutation (ALDH2*2) is a common frequent human gene variant, especially in East Asians. However, the expression and mechanism of action of ALDH2 in HNSC remain unknown. The present study explored the clinical significance and immune characteristics of ALDH2 in HNSC. The receiver operating characteristic curve was analysed to assess the diagnostic value of ALDH2 expression. ALDH2 expression in normal tissues and HNSC tissues was evaluated by IHC, and we also analysed ALDH2 gene expression in 4 HNSC cell lines. ALDH2 expression was significantly reduced in HNSC tissues compared to normal tissues (p < 0.05). HNSC patients with high ALDH2 expression had a better prognosis compared to patients with low ALDH2 expression (p < 0.05). GSEA indicated that these gene sets were correlated with signalling pathways, including the JAK-STAT signalling pathway. Unexpectedly, we found a significant prognostic effect of ALDH2 for HNSC based on alcohol consumption and the male sex. The correlation between ALDH2 expression and immune inhibitors showed an effect for ALDH2 in modifying tumour immunology in HNSC, and there may be a possible mechanism by which ALDH2 regulates the functions of T cells in HNSC. In addition, we developed a prognostic nomogram for HNSC patients, which suggested that low ALDH2 expression indicated poor prognosis in HNSC patients who were males and alcoholics.
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靶向单分子突变检测，并进行了大量平行测序。
影响因子：
14.9
作者：
Gregory MT;Bertout JA;Ericson NG;Taylor SD;Mukherjee R;Robins HS;Drescher CW;Bielas JH
通讯作者：
Bielas JH
影响因子：
28.2
作者：
Singh, Nathan;Lee, Yong Gu;Ruella, Marco
通讯作者：
Ruella, Marco
影响因子：
82.9
作者：
Binnewies M;Roberts EW;Kersten K;Chan V;Fearon DF;Merad M;Coussens LM;Gabrilovich DI;Ostrand-Rosenberg S;Hedrick CC;Vonderheide RH;Pittet MJ;Jain RK;Zou W;Howcroft TK;Woodhouse EC;Weinberg RA;Krummel MF
通讯作者：
Krummel MF
DOI：
10.1016/s1470-2045(14)71116-7
发表时间：
2015-04
期刊：
The Lancet. Oncology
影响因子：
--
作者：
Balachandran VP;Gonen M;Smith JJ;DeMatteo RP
通讯作者：
DeMatteo RP
影响因子：
4.7
作者：
Xie T;Guo X;Wu D;Li S;Lu Y;Wang X;Chen L
通讯作者：
Chen L