Development of cultivar-specific DNA markers based on retrotransposon-based insertional polymorphism in Japanese pear.
Development of cultivar-specific DNA markers based on retrotransposon-based insertional polymorphism in Japanese pear.
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DOI：
10.1270/jsbbs.62.53
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发表时间：
2012-03
期刊：
影响因子：
2.4
通讯作者：
Yamamoto T
中科院分区：
文献类型：
--
作者：
Kim H;Terakami S;Nishitani C;Kurita K;Kanamori H;Katayose Y;Sawamura Y;Saito T;Yamamoto T
我们开发了基于反转录转座子的插入多态性（RBIP）标记的基础上的copia类反转录转座子Ppcrt4和侧翼基因组序列的长末端重复序列（LTR）序列，这是来自454个测序数据，日本梨（梨）'丰水'。在包括LTR和侧翼基因组区域的40个序列中，我们开发了22个RBIP标记，并使用它们对80个梨品种进行DNA分析：64个日本梨品种、10个中国梨品种和6个欧洲梨品种。3个RBIP标记足以将丰水梨与其它日本梨品种区分开来。22个RBIP标记也能区分64个日本梨品种中的61个。欧洲梨的22个RBIP标记几乎没有扩增，这可能表明反转录转座子在亚洲梨进化过程中发生了转座，或者反映了亚洲梨和欧洲梨之间的遗传关系。16个RBIP标记可以定位在“穗”的遗传连锁图上。RBIP位点分布在10个连锁群中，有些位点在同一连锁群中的位置非常接近。所获得的信息将适用于开发植物中的品种特异性RBIP标记集。
We developed retrotransposon-based insertional polymorphism (RBIP) markers based on the long terminal repeat (LTR) sequences of copia-like retrotransposon Ppcrt4 and flanking genome sequences, which were derived from 454 sequencing data from Japanese pear (Pyrus pyrifolia) ‘Hosui’. Out of 40 sequences including both LTR and flanking genome regions, we developed 22 RBIP markers and used them for DNA profiling of 80 pear cultivars: 64 Japanese, 10 Chinese (Pyrus ussuriensis) and 6 European (Pyrus communis). Three RBIP markers were enough to differentiate ‘Hosui’ from the other Japanese pear cultivars. The 22 RBIP markers could also distinguish 61 of the 64 Japanese pear cultivars. European pears showed almost no amplification of the 22 RBIP markers, which might suggest that retrotransposons had transposed during Asian pear evolution or reflect the genetic relationship between Asian and European pears. Sixteen of the RBIP markers could be positioned on a genetic linkage map of ‘Hosui’. The RBIP loci were distributed in 10 linkage groups, and some loci were very closely located within the same linkage group. The information obtained will be applicable to developing cultivar-specific RBIP marker sets in plants.
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影响因子：
5.4
作者：
Diwan, N;Cregan, PB
通讯作者：
Cregan, PB
影响因子：
4
作者：
FLAVELL, AJ;PEARCE, SR;KUMAR, A
通讯作者：
KUMAR, A
DOI：
10.1080/14620316.2003.11511612
发表时间：
2003-03-01
影响因子：
1.9
作者：
Kimura, T;Iketani, H;Yamamoto, T
通讯作者：
Yamamoto, T
影响因子：
1.5
作者：
Kumar, A;Pearce, SR;Flavell, AJ
通讯作者：
Flavell, AJ
影响因子：
3.1
作者：
Vitte, C;Ishii, T;Panaud, O
通讯作者：
Panaud, O