Innate and Adaptive Cell-Mediated Immune Responses to a COVID-19 mRNA Vaccine in Young Children.
Innate and Adaptive Cell-Mediated Immune Responses to a COVID-19 mRNA Vaccine in Young Children.
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DOI：
10.1093/ofid/ofad608
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发表时间：
2023-12
影响因子：
4.2
通讯作者：
中科院分区：
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关于儿童对COVID-19 mRNA疫苗的细胞介导免疫（CMI）的信息很少。我们研究了6至60个月接种疫苗的儿童的适应性和先天性CMI。在第一剂之前和之后1个月从mRNA疫苗的随机安慰剂对照试验中的参与者获得的血液用于抗体测量和CMI（流式细胞术）。我们招募了29名儿童，平均年龄为28.5个月（SD，15.7）。抗体研究显示，10名参与者在接种前感染了SARS-CoV-2。用SARS-CoV-2刺突肽体外刺激外周血单核细胞显示，活化的常规CD 4+和γδ T细胞、自然杀伤细胞、单核细胞和常规树突状细胞在免疫前至免疫后显著增加，但粘膜相关先天性T细胞没有增加。常规T细胞，单核细胞和常规树突状细胞反应在接种疫苗后立即高于SARS-CoV-2感染后。接种疫苗前后CMI的倍数增加在先前感染SARS-CoV-2的儿童和未感染的儿童之间没有差异。接种COVID-19 mRNA疫苗的6至60个月的儿童产生了强大的CMI反应，包括适应性和先天免疫。
There is little information on cell-mediated immunity (CMI) to COVID-19 mRNA vaccines in children. We studied adaptive and innate CMI in vaccinated children aged 6 to 60 months. Blood obtained from participants in a randomized placebo-controlled trial of an mRNA vaccine before and 1 month after the first dose was used for antibody measurements and CMI (flow cytometry). We enrolled 29 children with a mean age of 28.5 months (SD, 15.7). Antibody studies revealed that 10 participants were infected with SARS-CoV-2 prevaccination. Ex vivo stimulation of peripheral blood mononuclear cells with SARS-CoV-2 spike peptides showed significant increases pre- to postimmunization of activated conventional CD4+ and γδ T cells, natural killer cells, monocytes, and conventional dendritic cells but not mucosa-associated innate T cells. Conventional T-cell, monocyte, and conventional dendritic cell responses in children were higher immediately after vaccination than after SARS-CoV-2 infection. The fold increase in CMI pre- to postvaccination did not differ between children previously infected with SARS-CoV-2 and those uninfected. Children aged 6 to 60 months who were vaccinated with a COVID-19 mRNA vaccine developed robust CMI responses, including adaptive and innate immunity.
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DOI：
10.3390/v13061031
发表时间：
2021-05-29
期刊：
Viruses
影响因子：
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作者：
Gaballa A;Alagrafi F;Uhlin M;Stikvoort A
通讯作者：
Stikvoort A
影响因子：
64.8
作者：
Liu J;Chandrashekar A;Sellers D;Barrett J;Jacob-Dolan C;Lifton M;McMahan K;Sciacca M;VanWyk H;Wu C;Yu J;Collier AY;Barouch DH
通讯作者：
Barouch DH
DOI：
10.1084/jem.20130417
发表时间：
2013-12-16
期刊：
The Journal of experimental medicine
影响因子：
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作者：
Firth MA;Madera S;Beaulieu AM;Gasteiger G;Castillo EF;Schluns KS;Kubo M;Rothman PB;Vivier E;Sun JC
通讯作者：
Sun JC
DOI：
10.1126/science.abm3425
发表时间：
2022-01-07
期刊：
Science (New York, N.Y.)
影响因子：
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TLR8：被遗忘的相对重生。
影响因子：
24.1
作者：
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