Regional Differences in Mucociliary Clearance in the Upper and Lower Airways.
Regional Differences in Mucociliary Clearance in the Upper and Lower Airways.
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DOI：
10.3389/fphys.2022.842592
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发表时间：
2022
影响因子：
4
通讯作者：
Grubb BR
中科院分区：
文献类型：
--
作者：
Rogers TD;Button B;Kelada SNP;Ostrowski LE;Livraghi-Butrico A;Gutay MI;Esther CR Jr;Grubb BR
由于鼻腔是哺乳动物中灵感的入口，因此必须将其浓缩的特定物质和病原体浓度，以使下呼吸道无菌，这可能会预计通过粘液层的速度（MCC）在该地区进行了更多的速度。鼻腔（后部鼻咽，PNP）比气管壁上大约是由于鼻腔中的纤毛含量更高的鼻腔（约80％）。后膜上的纤毛上皮似乎控制着表面结构，可能部分部分在我们的一个小鼠模型中的MCC（BPIFB1 KO）中的MCC速率在灌洗液中显示出3倍的MUC5B蛋白，在气管壁上的MCC颗粒传输严重损害，但正常的MCC在水管上均覆盖了Muc的毛毯。仅在PNP中。在气管中作为离散的斑块或粘液流。每个在上皮层中）。对比度，即使在气管中的小上皮漏洞上也无法运输颗粒。
As the nasal cavity is the portal of entry for inspired air in mammals, this region is exposed to the highest concentration of inhaled particulate matter and pathogens, which must be removed to keep the lower airways sterile. Thus, one might expect vigorous removal of these substances via mucociliary clearance (MCC) in this region. We have investigated the rate of MCC in the murine nasal cavity compared to the more distal airways (trachea). The rate of MCC in the nasal cavity (posterior nasopharynx, PNP) was ∼3–4× greater than on the tracheal wall. This appeared to be due to a more abundant population of ciliated cells in the nasal cavity (∼80%) compared to the more sparsely ciliated trachea (∼40%). Interestingly, the tracheal ventral wall exhibited a significantly lower rate of MCC than the tracheal posterior membrane. The trachealis muscle underlying the ciliated epithelium on the posterior membrane appeared to control the surface architecture and likely in part the rate of MCC in this tracheal region. In one of our mouse models (Bpifb1 KO) exhibiting a 3-fold increase in MUC5B protein in lavage fluid, MCC particle transport on the tracheal walls was severely compromised, yet normal MCC occurred on the tracheal posterior membrane. While a blanket of mucus covered the surface of both the PNP and trachea, this mucus appeared to be transported as a blanket by MCC only in the PNP. In contrast, particles appeared to be transported as discrete patches or streams of mucus in the trachea. In addition, particle transport in the PNP was fairly linear, in contrast transport of particles in the trachea often followed a more non-linear route. The thick, viscoelastic mucus blanket that covered the PNP, which exhibited ∼10-fold greater mass of mucus than did the blanket covering the surface of the trachea, could be transported over large areas completely devoid of cells (made by a breach in the epithelial layer). In contrast, particles could not be transported over even a small epithelial breach in the trachea. The thick mucus blanket in the PNP likely aids in particle transport over the non-ciliated olfactory cells in the nasal cavity and likely contributes to humidification and more efficient particle trapping in this upper airway region.
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DOI：
10.1126/science.1223012
发表时间：
2012-08-24
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Button B;Cai LH;Ehre C;Kesimer M;Hill DB;Sheehan JK;Boucher RC;Rubinstein M
通讯作者：
Rubinstein M
DOI：
10.1056/nejmra0910061
发表时间：
2010-12-02
期刊：
The New England journal of medicine
影响因子：
--
作者：
Fahy JV;Dickey BF
通讯作者：
Dickey BF
影响因子：
3.7
作者：
Karamaoun, Cyril;Sobac, Benjamin;Haut, Benoit
通讯作者：
Haut, Benoit
影响因子：
6.4
作者：
GABRIDGE, MG;AGEE, CC;CAMERON, AM
通讯作者：
CAMERON, AM
影响因子：
5.5
作者：
Grubb, Barbara R.;Rogers, Troy D.;Ostrowski, Lawrence E.
通讯作者：
Ostrowski, Lawrence E.