Direct release of angiotensins I and II from isolated rat kidney perfused with angiotensinogen-free medium.
Direct release of angiotensins I and II from isolated rat kidney perfused with angiotensinogen-free medium.
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从用不含血管紧张素原的培养基灌注的离体大鼠肾脏中直接释放血管紧张素 I 和 II。
DOI：
10.1016/0006-291x(87)90392-5
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发表时间：
1987
影响因子：
3.1
通讯作者：
Inagami,T
中科院分区：
文献类型：
--
作者：
Mizuno,K;Higashimori,K;Imada,T;Inagami,T
直接测量血管紧张素I和II免疫反应性物质的离体大鼠肾脏灌注液中的Krebs-Ringer溶液，这是免费的任何成分的肾素-血管紧张素系统。参照标准化合物，通过高压液相色谱法确认免疫反应性肽的身份为血管紧张素I和血管紧张素II。血管紧张素I和II在第一次灌注20 min内的释放速率分别高达1313.5 ± 184.5和772.4 ± 82.5 pg，并至少在2 h内保持稳定。在灌流液中同时测定的血管紧张素I分泌率和肾素分泌率之间以及血管紧张素I分泌率和血管紧张素II分泌率之间存在良好的相关性。这些结果与先前观察到的肾脏肾小球细胞中肾素和血管紧张素I和II的共存提供了肾内血管紧张素II形成和释放的证据。它不同意这些肽从循环中内化的观点。肾脏分泌的血管紧张素II可能在肾内调节中发挥多种功能。
A direct measurement of both angiotensins I and II immunoreactive substances was made in the perfusate from isolated rat kidney perfused with Krebs-Ringer solution which was free of any component of the renin-angiotensin system. The identity of the immunoreactive peptides was confirmed as angiotensin I and angiotensin II by high-pressure liquid chromatography in reference to standard compounds. The rate of release of angiotensins was as high as 1313.5 ± 184.5 and 772.4 ± 82.5 pg for angiotensins I and II, respectively, during the first perfusion period of 20 min, and it remained stable at least for 2 hours. There was a good relationship between the angiotensin I secretion rate and renin secretion rate simultaneously determined in the perfusate, and also between the angiotensin I secretion rate and angiotensin II secretion rate. These results taken together with the previous observations of the coexistence of renin and angiotensins I and II in juxtaglomerular cells of the kidney provide evidence for intrarenal formation and release of angiotensin II. It does not agree with the notion that these peptides are internalized from circulation. Angiotensin II secreted from the kidney may play diverse functions in intrarenal regulation.
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血管紧张素 II 在大鼠肾脏中局部存在及其通过膳食钠摄入和转化酶阻断进行调节的证据。
影响因子：
6
作者：
F. Mendelsohn
通讯作者：
F. Mendelsohn
人肾肾素的储存形式
影响因子：
8.3
作者：
M. Kawamura;S. Akabane;K. Ito;K. Ogino;C. Yutani;S. Go;M. Ikeda
通讯作者：
M. Ikeda
肾皮质的血管紧张素 I 转换酶。
DOI：
10.1016/0005-2744(74)90199-5
发表时间：
1974
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
G. Oshima;Á. Gecse;Ervin G. Erdös
通讯作者：
Ervin G. Erdös
实验性肾性高血压中缺血肾肾小球旁细胞中肾素和血管紧张素 II 的超微结构免疫细胞化学定位。
影响因子：
6
作者：
M. Cantin;J. Gutkowska;J. Lacasse;M. Ballak;S. Ledoux;T. Inagami;J. Beuzeron;J. Genest
通讯作者：
J. Genest
作为单层细胞培养物生长的肾小球旁细胞含有肾素、血管紧张素 I 转换酶以及血管紧张素 I 和 II/III
影响因子：
20.1
作者：
W. Rightsel;T. Okamura;T. Inagami;J. Pitcock;Y. Takii;B. Brooks;P. Brown;E. Muirhead
通讯作者：
E. Muirhead