Protein and small molecule recognition properties of deep cavitands in a supported lipid membrane determined by calcination-enhanced SPR spectroscopy.
Protein and small molecule recognition properties of deep cavitands in a supported lipid membrane determined by calcination-enhanced SPR spectroscopy.
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DOI：
10.1021/ja102252d
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发表时间：
2010-08-04
影响因子：
15
通讯作者：
Hooley, Richard J.
中科院分区：
文献类型：
--
作者：
Liu, Ying;Liao, Puhong;Cheng, Quan;Hooley, Richard J.
本报告详细介绍了在纳米玻璃表面组装的膜双层中加入可溶于水的深空穴的细节，用于在仿膜环境中研究分子识别。空穴和保留其宿主属性，并描述了通过表面等离子体共振和荧光显微镜实时分析膜：空穴和复合体的宿主：客体属性。基质对胆碱衍生的底物具有选择性，在膜双层中没有观察到底物的竞争性掺入。各种三甲基铵衍生的底物是合适的客体，显示出毫米级的结合亲和力。如果使用生物素衍生的客体分子，膜：空洞：客体复合体随后可以用来在膜表面捕获中性亲和素蛋白。中性亲和素的表面覆盖率受用于衍生生物素的间隔物的影响。增加与双层的距离可以使更高浓度的蛋白质被固定，这表明当结合事件从表面移开时，有害的空间位阻效应减弱。
This report details the incorporation of a water-soluble deep cavitand into a membrane bilayer assembled onto a nanoglassified surface for study of molecular recognition in a membrane-mimicking setting. The cavitand retains its host properties, and real-time analysis of the host:guest properties of the membrane:cavitand complex via surface plasmon resonance and fluorescence microscopy is described. The host shows selectivity for choline-derived substrates, and no competitive incorporation of substrate is observed in the membrane bilayer. A variety of trimethylammonium-derived substrates are suitable guests, displaying binding affinities in a millimolar range. The membrane:cavitand:guest complexes can be subsequently used to capture NeutrAvidin protein at the membrane surface if a biotin-derived guest molecule is used. The surface coverage of NeutrAvidin is affected by the spacer used to derivatize the biotin. Increased distance from the bilayer allows a higher concentration of protein to be immobilized, suggesting a diminishing detrimental steric effect when the binding event is shifted away from the surface.
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影响因子：
7.4
作者：
Guerrini, Luca;Garcia-Ramos, Jose V.;Sanchez-Cortes, Santiago
通讯作者：
Sanchez-Cortes, Santiago
影响因子：
15
作者：
Biros, SM;Ullrich, EC;Rebek, J
通讯作者：
Rebek, J
影响因子：
15
作者：
Jakobsen, Ulla;Simonsen, Adam C.;Vogel, Stefan
通讯作者：
Vogel, Stefan
影响因子：
16.6
作者：
Hooley, Richard J.;Biros, Shannon M.;Rebek, Julius, Jr.
通讯作者：
Rebek, Julius, Jr.
影响因子：
15
作者：
Boonyarattanakalin, Siwarutt;Hu, Jianfang;Peterson, Blake R.
通讯作者：
Peterson, Blake R.