Time-evolving genetic networks reveal a NAC troika that negatively regulates leaf senescence in Arabidopsis.
Time-evolving genetic networks reveal a NAC troika that negatively regulates leaf senescence in Arabidopsis.
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DOI：
10.1073/pnas.1721523115
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发表时间：
2018-05-22
影响因子：
11.1
通讯作者：
Hwang D
中科院分区：
文献类型：
--
作者：
Kim HJ;Park JH;Kim J;Kim JJ;Hong S;Kim J;Kim JH;Woo HR;Hyeon C;Lim PO;Nam HG;Hwang D
叶片衰老的调控是一个复杂的方式，涉及与发育和环境信号的时间依赖性的相互作用。遗传筛选已经确定了衰老的关键调节因子，特别是晚期衰老调节因子。最近，时间过程基因表达和网络分析，主要是静态网络的分析，预测了许多衰老调节因子。然而，衰老是由时间演化网络定义的，涉及衰老调节因子之间相互作用的时间转变。在这里，我们提出了NAM/ATAF/CUC（NAC）转录因子，在拟南芥叶片衰老的中央调节器，通过时程基因表达分析的NAC在其突变体的时间演变网络。这些随时间演变的网络揭示了一个独特的调控模块的NAC，控制及时诱导衰老促进过程中的早衰阶段的叶老化。衰老是由时间演化网络控制的，该网络描述了衰老调节因子之间相互作用的时间转变。在这里，我们提出了在拟南芥叶片老化过程中NAM/ATAF/CUC（NAC）转录因子的时间演化网络。这些时间依赖性网络的最明显的特征是在衰老阶段NACs之间从正调控转变为负调控。ANAC 017、ANAC 082和ANAC 090被称为“NAC三驾马车”，负责管理从积极到消极的监管转变。NAC三驾马车的敲除加速衰老和其他NAC的诱导，而NAC三驾马车的过表达具有相反的效果。转录组和分子分析揭示了NAC三驾马车对衰老促进过程的共同抑制，包括水杨酸（SA）和活性氧（ROS）反应，但分别由ANAC 090和ANAC 017对SA和ROS反应进行主要调节。我们的时间进化网络提供了一个独特的早老抑制因子的调节模块，该模块在叶衰老的早老阶段指导衰老促进过程的及时诱导。
Leaf senescence is regulated in a complex manner, involving time-dependent interactions with developmental and environmental signals. Genetic screens have identified key regulators of senescence, particularly late-stage senescence regulators. Recently, time-course gene-expression and network analyses, mostly analyses of static networks, have predicted many senescence regulators. However, senescence is defined by time-evolving networks, involving the temporal transition of interactions among senescence regulators. Here, we present time-evolving networks of NAM/ATAF/CUC (NAC) transcription factors, central regulators of leaf senescence in Arabidopsis, via time-course gene-expression analysis of NACs in their mutants. These time-evolving networks revealed a unique regulatory module of NACs that controls the timely induction of senescence-promoting processes at a presenescent stage of leaf aging. Senescence is controlled by time-evolving networks that describe the temporal transition of interactions among senescence regulators. Here, we present time-evolving networks for NAM/ATAF/CUC (NAC) transcription factors in Arabidopsis during leaf aging. The most evident characteristic of these time-dependent networks was a shift from positive to negative regulation among NACs at a presenescent stage. ANAC017, ANAC082, and ANAC090, referred to as a “NAC troika,” govern the positive-to-negative regulatory shift. Knockout of the NAC troika accelerated senescence and the induction of other NACs, whereas overexpression of the NAC troika had the opposite effects. Transcriptome and molecular analyses revealed shared suppression of senescence-promoting processes by the NAC troika, including salicylic acid (SA) and reactive oxygen species (ROS) responses, but with predominant regulation of SA and ROS responses by ANAC090 and ANAC017, respectively. Our time-evolving networks provide a unique regulatory module of presenescent repressors that direct the timely induction of senescence-promoting processes at the presenescent stage of leaf aging.
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