Discovery of a Novel Species of Trichomonasvirus in the Human Parasite Trichomonas vaginalis Using Transcriptome Mining.
Discovery of a Novel Species of Trichomonasvirus in the Human Parasite Trichomonas vaginalis Using Transcriptome Mining.
复制标题
DOI：
10.3390/v14030548
复制
发表时间：
2022-03-06
期刊：
影响因子：
--
通讯作者：
Nibert ML
中科院分区：
文献类型：
--
作者：
Manny AR;Hetzel CA;Mizani A;Nibert ML
阴道毛滴虫是全球最常见的性传播感染的非病毒性原因。这种原生动物寄生虫的感染导致滴虫病临床综合征，表现为急性和慢性后果的炎症性疾病。这种寄生虫的一半或更多分离株本身感染了一种或多种可加重炎症综合征的dsRNA病毒。迄今为止，在阴道滴虫中已鉴定出至少四种不同的病毒，包括滴虫病毒属的阴道滴虫病毒1种和阴道滴虫病毒4种。尽管这些病毒在全球流行，但很少有完整的编码序列被报道。我们对60个RNA-Seq数据库中公开可用的转录组进行了病毒序列挖掘，这些转录组代表了至少13种不同的阴道T.分离株。结果产生了27个新的毛滴虫病毒株的序列组装，涵盖所有4个已识别的物种。利用从头序列组装和分类分类的策略，我们还发现了新发现的第五种的6株，我们建议将其命名为阴道毛滴虫病毒5，也属于毛滴虫病毒属。这些额外的菌株彼此具有较高的序列同源性，但与其他四种菌株的序列同源性较低。系统发育分析证实了物种水平的指定。这些结果大大增加了毛滴虫病毒基因组序列的数量，并证明了在关键人类病原体中挖掘公开可用转录组的效用。
Trichomonas vaginalis is the most common non-viral cause of sexually transmitted infections globally. Infection by this protozoan parasite results in the clinical syndrome trichomoniasis, which manifests as an inflammatory disease with acute and chronic consequences. Half or more isolates of this parasite are themselves infected with one or more dsRNA viruses that can exacerbate the inflammatory syndrome. At least four distinct viruses have been identified in T. vaginalis to date, constituting species Trichomonas vaginalis virus 1 through Trichomonas vaginalis virus 4 in genus Trichomonasvirus. Despite the global prevalence of these viruses, few complete coding sequences have been reported. We conducted viral sequence mining in publicly available transcriptomes across 60 RNA-Seq accessions representing at least 13 distinct T. vaginalis isolates. The results led to sequence assemblies for 27 novel trichomonasvirus strains across all four recognized species. Using a strategy of de novo sequence assembly followed by taxonomic classification, we additionally discovered six strains of a newly identified fifth species, for which we propose the name Trichomonas vaginalis virus 5, also in genus Trichomonasvirus. These additional strains exhibit high sequence identity to each other, but low sequence identity to strains of the other four species. Phylogenetic analyses corroborate the species-level designations. These results substantially increase the number of trichomonasvirus genome sequences and demonstrate the utility of mining publicly available transcriptomes for virus discovery in a critical human pathogen.
登录
查看更多内容
影响因子：
3.2
作者：
Fraga, Jorge;Rojas, Lazara;Fernandez-Calienes, Ayme
通讯作者：
Fernandez-Calienes, Ayme
影响因子：
9.2
作者：
Bushmanova, Elena;Antipov, Dmitry;Prjibelski, Andrey D.
通讯作者：
Prjibelski, Andrey D.
影响因子：
6.4
作者：
Chiba, Sotaro;Jamal, Atif;Suzuki, Nobuhiro
通讯作者：
Suzuki, Nobuhiro
影响因子：
3.7
作者：
Bessarab, IN;Liu, HW;Tai, JH
通讯作者：
Tai, JH
影响因子：
10.7
作者：
Nguyen LT;Schmidt HA;von Haeseler A;Minh BQ
通讯作者：
Minh BQ