In vivo (31)P NMR spectroscopy assessment of skeletal muscle bioenergetics after spinal cord contusion in rats.
In vivo (31)P NMR spectroscopy assessment of skeletal muscle bioenergetics after spinal cord contusion in rats.
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DOI：
10.1007/s00421-013-2810-9
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发表时间：
2014-04
影响因子：
3
通讯作者：
Vandenborne, Krista
中科院分区：
文献类型：
--
作者：
Shah, Prithvi K.;Ye, Fan;Liu, Min;Jayaraman, Arun;Baligand, Celine;Walter, Glenn;Vandenborne, Krista
Muscle paralysis after spinal cord injury (SCI) leads to muscle atrophy, enhanced muscle fatigue, and increased energy demands for functional activities. Phosphorus magnetic resonance spectroscopy (31P-MRS) offers a unique non-invasive alternative of measuring energy metabolism in skeletal muscle and is especially suitable for longitudinal investigations. We determined the impact of spinal cord contusion on in-vivo muscle bioenergetics of the rat hindlimb muscle using 31P-MRS. A moderate spinal cord contusion injury (cSCI) was induced at the T8-T10 thoracic spinal segments. 31P-MRS measurements were performed weekly in the rat hindlimb muscles for three weeks. Spectra were acquired in a Bruker 11T/470 MHz spectrometer using a 31P surface coil. The sciatic nerve was electrically stimulated by subcutaneous needle electrodes. Spectra were acquired at rest (5 min), during stimulation (6 min), and recovery (20 min). Phosphocreatine (PCr) depletion rates and the pseudo-first-order rate constant for PCr recovery (kPCr) were determined. The maximal rate of PCr resynthesis, the in-vivo maximum oxidative capacity (Vmax) and oxidative ATP synthesis rate (Qmax), were subsequently calculated. One week after cSCI, there was a decline in the resting [TCr] of the paralyzed muscle. There was a significant reduction (~24%) in kPCr measures of the paralyzed muscle, maximum in-vivo mitochondrial capacity (Vmax) and the maximum oxidative ATP synthesis rate (Qmax) at 1week post-cSCI. Using in-vivo MRS assessments, we reveal an acute oxidative metabolic defect in the paralyzed hind limb muscle. These altered muscle bioenergetics might contribute to the host of motor dysfunctions seen after cSCI.
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影响因子：
1.7
作者：
Johnston, TE;Finson, RL;Mulcahey, MJ
通讯作者：
Mulcahey, MJ
影响因子：
3.3
作者：
Kent-Braun, JA;Ng, AV
通讯作者：
Ng, AV
影响因子：
3.3
作者：
Hayashi, Y;Ikata, T;Koga, K
通讯作者：
Koga, K
影响因子：
2.4
作者：
Argov, Z;Arnold, DL
通讯作者：
Arnold, DL
DOI：
10.1152/ajpheart.2001.281.2.h882
发表时间：
2001-08-01
影响因子：
4.8
作者：
Hitchins, S;Cieslar, JM;Dobson, GP
通讯作者：
Dobson, GP