3H-31, A Non-structural Protein of Heliothis virescens ascovirus 3h, Inhibits the Host Larval Cathepsin and Chitinase Activities
3H-31, A Non-structural Protein of Heliothis virescens ascovirus 3h, Inhibits the Host Larval Cathepsin and Chitinase Activities
复制标题
3H-31，烟夜蛾子囊病毒的非结构蛋白 3h，抑制宿主幼虫组织蛋白酶和几丁质酶活性
DOI：
10.1007/s12250-021-00374-y
复制
发表时间：
2021-04
影响因子：
5.5
通讯作者：
Guo-Hua Huang
中科院分区：
文献类型：
--
作者：
Huan Yu;Yi-Yi Ou-Yang;Chang-Jin Yang;Ni Li;Madoka Nakai;Guo-Hua Huang
烟芽夜蛾（Heliothis virescens）的3 h-31基因（HvAV-3 h）是一个高度保守的基因。3 h-31作为HvAV-3 h的早期基因，编码HvAV-3 h的非结构蛋白（3 H-31）。在该研究中，3 h-31最初被转录，并在感染后3 h（hpi）在感染的甜菜夜蛾脂肪体细胞（SeFB）中表达。3 h-31进一步插入苜蓿银纹夜蛾核型多角体病毒（AcMNPV）的杆粒中，获得了具有感染性的杆状病毒。（AcMNPV-31）。体内实验表明，随着3 H-31基因的表达，AcMNPV-31感染的SeFB细胞中出芽病毒的产生和病毒DNA的复制减少，病毒基质周围出现透明的管状结构。AcMNPV-31对三龄棉铃虫的LD_（50）和LD_（90）均显著高于野生型AcMNPV（AcMNPV-wt）。甜菜幼虫AcMNPV-31感染致死的幼虫的液化时间延迟。AcMNPV-31感染幼虫的几丁质酶和组织蛋白酶活性显著低于AcMNPV-wt感染幼虫。利用RNAi技术进一步证实了几丁质酶和组织蛋白酶对3 h-31的可能调控作用，表明3 h-31的RNAi显著上调了幼虫的组织蛋白酶活性，但几丁质酶活性没有显著变化。根据上述结果，我们推测3 H-31的作用可能与抑制寄主幼虫的几丁质酶和组织蛋白酶活性有关，从而在幼虫期抑制寄主。
3h-31 of Heliothis virescens ascovirus 3h (HvAV-3h) is a highly conserved gene of ascoviruses. As an early gene of.HvAV-3h, 3h-31 codes for a non-structural protein (3H-31) of HvAV-3h. In the study, 3h-31 was initially transcribed and.expressed at 3 h post-infection (hpi) in the infected Spodoptera exigua fat body cells (SeFB). 3h-31 was further inserted.into the bacmid of Autographa californica nucleopolyhedrovirus (AcMNPV) to generate an infectious baculovirus.(AcMNPV-31). In vivo experiments showed that budded virus production and viral DNA replication decreased with the.expression of 3H-31, and lucent tubular structures were found around the virogenic stroma in the AcMNPV-31-infected.SeFB cells. In vivo, both LD50 and LD90 values of AcMNPV-31 were significantly higher than those of the wild-type.AcMNPV (AcMNPV-wt) in third instar S. exigua larvae. An interesting finding was that the liquefaction of the larvae.killed by the infection of AcMNPV-31 was delayed. Chitinase and cathepsin activities of AcMNPV-31-infected larvae.were significantly lower than those of AcMNPV-wt-infected larvae. The possible regulatory function of the chitinase and.cathepsin for 3H-31 was further confirmed by RNAi, which showed that larval cathepsin activity was significantly.upregulated, but chitinase activity was not significantly changed due to the RNAi of 3h-31. Based on the obtained results,.we assumed that the function of 3H-31 was associated with the inhibition of host larval chitinase and cathepsin activities,.so as to restrain the hosts in their larval stages.
登录
查看更多内容
DOI：
10.1016/b978-012374410-4.00432-5
发表时间：
2008
期刊：
--
影响因子：
--
作者：
M. Erlandson
通讯作者：
M. Erlandson
OfCht5（来自昆虫玉米螟的几丁质酶）的克隆、表达和生物表征
影响因子：
4
作者：
Wu, Qingyue;Liu, Tian;Yang, Qing
通讯作者：
Yang, Qing
烟夜蛾子囊病毒3h(HvAV-3h)感染三种主要农业害虫生长发育比较
影响因子：
3.7
作者：
Li SJ;Wang X;Zhou ZS;Zhu J;Hu J;Zhao YP;Zhou GW;Huang GH
通讯作者：
Huang GH
通过转录组分析宿主甜菜夜蛾幼虫对烟夜蛾子囊病毒3h（HvAV-3h）感染的反应
影响因子：
4.6
作者：
Yu H;Li ZQ;He L;Ou-Yang YY;Li N;Huang GH
通讯作者：
Huang GH
改变宿主幼虫（甜菜夜蛾）钙调神经磷酸酶活性以响应子囊病毒感染
影响因子：
4.1
作者：
Huan Yu;Lei He;Zi-Qi Li;Ni Li;Yi-Yi Ou-Yang;Guo-Hua Huang
通讯作者：
Guo-Hua Huang