A comparison of growth and development of three major agricultural insect pests infected with Heliothis virescens ascovirus 3h (HvAV-3h).
A comparison of growth and development of three major agricultural insect pests infected with Heliothis virescens ascovirus 3h (HvAV-3h).
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烟夜蛾子囊病毒3h(HvAV-3h)感染三种主要农业害虫生长发育比较
DOI：
10.1371/journal.pone.0085704
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Huang GH
中科院分区：
文献类型：
--
作者：
Li SJ;Wang X;Zhou ZS;Zhu J;Hu J;Zhao YP;Zhou GW;Huang GH
子囊病毒是一种双链DNA病毒，对鳞翅目宿主，特别是夜蛾幼虫具有致病性。幼虫感染的特点是生长迟缓，摄食减少，体色变黄。本文报道了三种主要的农业夜蛾害虫——棉铃虫（Helicoverpa armigera, h<s:1> bner）、夜蛾（Spodoptera exigua, h<s:1> bner）和斜纹夜蛾（Spodoptera litura, Fabricius）在感染Heliothis virescens ascovirus 3h （HvAV-3h）后的生长发育情况。用含hvav -3h的血淋巴连续稀释10倍（0 ~ 7）感染斜纹夜蛾幼虫，0 ~ 103倍稀释对幼虫死亡率无显著影响；然而，在104倍及以上稀释时观察到显著差异。将含hvav -3h的血淋巴稀释10倍后，分别感染棉铃虫、夜蛾和斜纹夜蛾幼虫，发现其生长发育受到明显影响。所有感染的幼虫均不能化蛹；处理后的棉铃虫、斜纹夜蛾和夜蛾幼虫的存活时间明显长于未处理的对照幼虫。对照组与对照组的体重在接种后第1天差异显著，在接种后第2天差异显著。此外，接种后第2天，棉铃虫和斜纹夜蛾的摄取量与未接种对照组的摄取量也有显著差异，而接种后第3天，甜菜夜蛾的摄取量则有显著差异。
Ascoviruses are double-stranded DNA viruses that are pathogenic to lepidopteran hosts, particularly noctuid larvae. Infection of a larva is characterized by retarded growth, reduced feeding and yellowish body color. In this paper, we reported the growth and development of three major agricultural noctuid insect pests, Helicoverpa armigera (Hübner), Spodoptera exigua (Hübner) and Spodoptera litura (Fabricius), infected with Heliothis virescens ascovirus 3h (HvAV-3h). Using 10-fold serial dilutions (0 to 7) of HvAV-3h-containing hemolymph to infect S. litura larvae, we found no significant difference in larval mortalities from 0 to 103-fold dilutions; however, significant differences were observed at 104-fold dilution and above. Using a 10-fold dilution of HvAV-3h-containing hemolymph to infect H. armigera, S. exigua and S. litura larvae, we found that the growth and development were significantly affected. All infected larvae could not pupate; the survival times of treated H. armigera, S. litura and S. exigua larvae were significantly longer than untreated control larvae. Body weight showed significant difference between treated and untreated control group from day 1 after inoculation in H. armigera and S. exigua, but day 2 in S. litura. Additionally, food intake also showed significant difference between treated and untreated control group from day 2 after inoculation in H. armigera and S. litura, but day 3 in S. exigua.
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影响因子：
3.8
作者：
Cheng, XW;Carner, GR;Arif, BM
通讯作者：
Arif, BM
影响因子：
3.8
作者：
Cheng, XW;Carner, GR;Brown, TM
通讯作者：
Brown, TM
影响因子：
5.4
作者：
de Jong, Jondavid;Arif, Basil M.;Krell, Peter J.
通讯作者：
Krell, Peter J.
影响因子：
3.8
作者：
FEDERICI, BA;VLAK, JM;HAMM, JJ
通讯作者：
HAMM, JJ
DOI：
10.1073/pnas.80.24.7664
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
FEDERICI, BA
通讯作者：
FEDERICI, BA