Simultaneous monitoring of independent gene expression patterns in two types of cocultured fibroblasts with different color-emitting luciferases.
Simultaneous monitoring of independent gene expression patterns in two types of cocultured fibroblasts with different color-emitting luciferases.
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DOI：
10.1186/1472-6750-8-40
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发表时间：
2008-04-17
影响因子：
3.5
通讯作者：
Nakajima Y
中科院分区：
文献类型：
--
作者：
Noguchi T;Ikeda M;Ohmiya Y;Nakajima Y
荧光素酶测定系统能够实时监测活细胞中的基因表达。我们已经开发了一种双色荧光素酶检测系统，其中多个基因的表达可以同时跟踪使用绿色和红色发光甲虫的酶。我们已经应用该系统监测独立的基因表达在两种类型的共培养成纤维细胞在真实的时间。建立了两种Rat-1细胞系，其在典型的时钟基因mBmal 1启动子的控制下稳定表达绿色或红色发光酶。我们共培养这些细胞系，同时监测两者的基因表达谱。这些细胞系的昼夜节律是独立的，即使在共培养时也遵循其固有的昼夜节律阶段振荡。此外，独立的节奏同步介质的变化作为一种外部刺激。使用这个系统，我们成功地监测独立的基因表达模式，在两行共培养的成纤维细胞。
Luciferase assay systems enable the real-time monitoring of gene expression in living cells. We have developed a dual-color luciferase assay system in which the expression of multiple genes can be tracked simultaneously using green- and red-emitting beetle luciferases. We have applied the system to monitoring independent gene expressions in two types of cocultured fibroblasts in real time. Two Rat-1 cell lines were established that stably express either green- or red-emitting luciferases under the control of the mBmal1 promoter, a canonical clock gene. We cocultured these cell lines, and gene expression profiles in both were monitored simultaneously. The circadian rhythms of these cell lines are independent, oscillating following their intrinsic circadian phases, even when cocultured. Furthermore, the independent rhythms were synchronized by medium change as an external stimulus. Using this system, we successfully monitored independent gene expression patterns in two lines of cocultured fibroblasts.
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影响因子：
16.2
作者：
WELSH, DK;LOGOTHETIS, DE;REPPERT, SM
通讯作者：
REPPERT, SM
影响因子：
5.6
作者：
Nueda, Maria-Luisa;Baladron, Victoriano;Laborda, Jorge
通讯作者：
Laborda, Jorge
DOI：
10.1073/pnas.52.1.75
发表时间：
1964-01-01
影响因子：
11.1
作者：
SELIGER, HH;MCELROY, WD
通讯作者：
MCELROY, WD
影响因子：
4.8
作者：
Su, Gui;Blaine, Stacy A.;Friedl, Andreas
通讯作者：
Friedl, Andreas
影响因子：
56.9
作者：
Balsalobre, A;Brown, SA;Schibler, U
通讯作者：
Schibler, U