Semi-chronic laminar recordings in the brainstem of behaving marmoset monkeys
Semi-chronic laminar recordings in the brainstem of behaving marmoset monkeys
复制标题
行为狨猴脑干的半慢性层流记录
DOI：
10.1016/j.jneumeth.2018.10.026
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发表时间：
2019
影响因子：
3
通讯作者：
Hage SR
中科院分区：
文献类型：
--
作者：
Pomberger T;Hage SR
背景多电极阵列慢性记录被广泛用于研究灵长类动物复杂行为背后的神经网络。这些系统中的大多数被设计用于研究皮质半球中的神经活动，导致缺乏能够同时记录深层脑干结构中的神经元集合的设备。然而，要充分了解复杂的行为，它是根本也破译的内在机制的潜在的运动模式产生电路在brainstem.New methodWe报告一个轻量级的系统，同时测量单单位活动从大量的记录网站在脑干的绒猴。它包括一个固定在动物头骨上的底室和一个可移动的上室，上室可以半慢性地安装在底室上，以灵活地定位一个嵌入式微驱动器，该微驱动器包含一个32通道层流探针，以从脑干内的各个位置记录数周。从发声的绒猴脑干中大量记录位点的单位活动。与现有方法的比较据我们所知，在清醒时用多位点层状探针从脑干深部结构记录的慢性系统，行为猴子还不存在。ConclusionsThe半慢性植入层状电极到行为的绒猴脑干打开了新的研究可能性，在充分了解复杂的行为在绒猴的神经机制。
BackgroundChronic recordings with multi-electrode arrays are widely used to study neural networks underlying complex primate behaviors. Most of these systems are designed for studying neural activity in the cortical hemispheres resulting in a lack of devices being capable of simultaneously recording from ensembles of neurons in deep brainstem structures. However, to fully understand complex behavior, it is fundamental to also decipher the intrinsic mechanisms of the underlying motor pattern generating circuits in the brainstem.New methodWe report a light-weight system that simultaneously measures single-unit activity from a large number of recording sites in the brainstem of marmoset monkeys. It includes a base chamber fixed to the animal’s skull and a removable upper chamber that can be semi-chronically mounted to the base chamber to flexibly position an embedded micro-drive containing a 32-channel laminar probe to record from various positions within the brainstem for several weeks.ResultsThe current system is capable of simultaneously recording stable single-unit activity from a large number of recording sites in the brainstem of vocalizing marmoset monkeys.Comparison with existing methodsTo the best of our knowledge, chronic systems to record from deep brainstem structures with multi-site laminar probes in awake, behaving monkeys do not yet exist.ConclusionsThe semi-chronic implantation of laminar electrodes into the brainstem of behaving marmoset monkeys opens new research possibilities in fully understanding the neural mechanisms underlying complex behaviors in marmoset monkeys.
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影响因子：
3.7
作者：
Roberts, AC;Wallis, JD
通讯作者：
Wallis, JD
影响因子：
16.6
作者：
Gultekin YB;Hage SR
通讯作者：
Hage SR
狨猴（Callithrix jacchus）纹状皮层的视觉组织
DOI：
--
发表时间：
1996
期刊：
The Journal of comparative neurology
影响因子：
--
作者：
K. Fritsches;M. Rosa
通讯作者：
M. Rosa
DOI：
10.1038/s41583-018-0003-6
发表时间：
2018-06
期刊：
Nature reviews. Neuroscience
影响因子：
--
作者：
Del Negro CA;Funk GD;Feldman JL
通讯作者：
Feldman JL
影响因子：
13.6
作者：
Gultekin YB;Hage SR
通讯作者：
Hage SR